	   SnapGene      šACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCCTCGTTCATTCACGTTTTTGAACCCGTGGAGGACGGGCAGACTCGCGGTGCAAATGTGTTTTACAGCGTGATGGAGCAGATGAAGATGCTCGACACGCTGCAGAACACGCAGCTAGATTAACCCTAGAAAGATAATCATATTGTGACGTACGTTAAAGATAATCATGCGTAAAATTGACGCATGTGTTTTATCGGTCTGTATATCGAGGTTTATTTATTAATTTGAATAGATATTAAGTTTTATTATATTTACACTTACATACTAATAATAAATTCAACAAACAATTTATTTATGTTTATTTATTTATTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAACAAAACTTTTATGAGGGACAGCCCCCCCCCAAAGCCCCCAGGGATGTAATTACGTCCCTCCCCCGCTAGGGGGCAGCAGCGAGCCGCCCGGGGCTCCGCTCCGGTCCGGCGCTCCCCCCGCATCCCCGAGCCGGCAGCGTGCGGGGACAGCCCGGGCACGGGGAAGGTGGCACGGGATCGCTTTCCTCTGAACGCTTCTCGCTGCTCTTTGAGCCTGCAGACACCTGGGGGGATACGGGGAAAAGGCCTCCAAGGCCTACTAGTCCCTTTCGTCTTCACTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGAAGAGTTTACTCCCTATCAGTGATAGAGAACGTATGCAGACTTTACTCCCTATCAGTGATAGAGAACGTATAAGGAGTTTACTCCCTATCAGTGATAGAGAACGTATGACCAGTTTACTCCCTATCAGTGATAGAGAACGTATCTACAGTTTACTCCCTATCAGTGATAGAGAACGTATATCCAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGGTAGGCGTGTACGGTGGGCGCCTATAAAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGCAATTCCACAACACTTTTGTCTTATACTTGGATCCGGGCCCGCGGCCGCCCCGGGACGCGTCATATGGAATTCTACCTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCAGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAGTGACACTTACCGCATTGACAAGCACGCCTCACGGGAGCTCCAAGCGGCGACTGAGATGTCCTAAATGCACAGCGACGGATTCGCGCTATTTAGAAAGAGAGAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATCTTTCTAGGGTTAATCTAGCTGCATCAGGATCATATCGTCGGGTCTTTTTTCCGGCTCAGTCATCGCCCAAGCTGGCGCTATCTGGGCATCGGGGAGGAAGAAGCCCGTGCCTTTTCCCGCGAGGTTGAAGCGGCATGGAAAGAGTTTGCCGAGGATGACTGCTGCTGCATTGACGTTGAGCGAAAACGCACGTTTACCATGATGATTCGGGAAGGTGTGGCCATGCACGCCTTTAACGGTGAACTGTTCGTTCAGGCCACCTGGGATACCAGTTCGTCGCGGCTTTTCCGGACACAGTTCCGGATGGTCAGCCCGAAGCGCATCAGCAACCCGAACAATACCGGCGACAGCCGGAACTGCCGTGCCGGTGTGCAGATTAATGACAGCGGTGCGGCGCTGGGATATTACGTCAGCGAGGACGGGTATCCTGGCTGGATGCCGCAGAAATGGACATGGATACCCCGTGAGTTACCCGGCGGGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCAT  +5               
         
               "                              
                                          
                                                                                          
                     	   !   
                           &   8                                                                        #            	               
                        	                                                               
      "                  "                                          $   *                                                    
                                 	                        (                                                         
            
         	                                                         
                     ^ Àš›�‘¥Úë./08	sõ©å §ÁàhœZtÛìù19ÚO	�	Æ	N
FtöóÎž=¨u $ _ ü €¶ÁÆçd
ªO >RôüBmz’¦åsÉ	�
‰;¢þæ}
 ¨ï8Ð¢P	uŸ œÂÜáií:è	H	‘	 
ð
G&¬ô)ºú>×^v† 7 �uú2Ãý{ÚX	Ç	í	O
q
†
÷Ù²‰Ïê©ö
 .[ÜÛá	`
€
Žý ‡uñp•	­	.
C
}#Ìâ\ƒ_P ·ce
ÇÜl†«Á5V– % ` ý Â¿5zÇ:	}ä_«à �±²èñ	²
²)«Ö2í" ?SÊÓõýCn“ª·gt\		
H
š
ž
§
zŠÀÙ?€ŸüÉ»‡�ç~ Õ “Ðæô	C	žDQ{%b Y§ôØ	m
ºM<ð£Ø› Ë Y{á@IÊÑš	¾	h!3å¯ÿ
 …½Xa	8	V	^
x{¿O  ö Ö| Äè0Tc�‘vÜÈx e‰ÑâM	‘
{Õ0(F¾bŽ 3 ©·ðè9œË„£Q“q	ë
Ã^»S  ÝâjAî† 	#	>	’	ú	ñ
­õÝú’û
? _ N t Ï BÃØåë–n;¢Ê‹I	u	{
Ç
Hƒ*�¿Øê‡
 C í Ðé¡cïzw� (e�ÍV	~¢Æê2V‘Ä°	
!
'H»À Vž5>DveY	ˆ	î	r
N 8 ø r�Õ3xœÀä,PþýÈ	P
‡
ø˜Ú�™Àª ±­c|‹	6
³vÐÞß qûšÄÛ?Š•�ë÷`µÇõ÷, ½ÑÞ€ Üxk	a
[n° K\Ý'0?â	
=
�
Š‹þ7� /­_¹\	¤��€[ ò òÉZÊŠ ,Ñ	òqûx˜	~úàã]¸� cæ3~vš¾â*N�.®	�
Å
MY`Q '§j–	/
Ã
û
$@‹„ y ˆv¡Ö*	)
1
D
	R¸BÍ¶ÔË 
 
dÁ&Æ6W�L ‡ �¸×˜Hy½÷ª
ý
Ý,m‡¬*HJ G ¶‚¦Êî6Z�Þf
„ÈÂ	 —� ƒld þ e³Ôu;	n	+92y7ilÑù�Œ & � ˜ ¤ ¯ ‡6µäÈ“
Í
å É;WÀá  xÃý)©ÀÝg‹Ó÷?Š"{O		¼
~B¢`h^’I a ó#µ	¹	Ï	è	¶Ä-Q×nO¬Þ ‚^äÓ	CO³*ˆ� 8Ì–áò«úò	X
³
w×†ü>p  , J²[¸év¬3e, H�³Rhá}î 5 òxØÏZA	Ö	A
F
›
q{¾ÅÚÊZÞ… @TËÔéo”«š]v6	s	
\
Á�½^Æ¼ˆè ‹ ¢ F1–Ôhß©¨
)‚7@ øžG f öþ.7D]êì¸u�
	ý	I
Ÿ
¡
.o‹¬ºýa|b³ ;S\”—Ñ$ª�)¶3
x
þŸ¢¸ps~&½ Ö _¦!¥B•
El»èdÖUcm  £ÃH_m:¥Ô	D	f	ÿ	ô
[`|.4 h ’çõDq{	 	DR)"  Z�£PÑ	`	c	K
n
¹
X©»<8=^S¸  ø<„_Œöƒ	ÏñBu ¹0ÿ,Ù	£
;•ÒÿD¤œQ ¸ G¨­º†SõÔ
0��®ÎNq°¼
19³ÑÙ‹¦å“Í Ì ŠÇ+9žÍÒS	
&
+
u
í
4Ï÷ :j { QŒ½â›	¿	Ti"˜¥óSoCµ |î AJwžH,	¤	åcæ|! Z«`m¡áËÕ 	&	ö	7SV°™ # ] û =Q¦ßûAl™ÀYs�ÅôÍ¡ýt| ÛîÏÙ	W	_
 %�±üˆéõ]
 †¾ðb9	”	|(À Ê X©?Hf½	yØ 2LžÈPzºa ¶{ˆŸÃç/SƒŽ�’L	wzºÔ/E M ì '×äUm}¡Åé1U¡ÃŠ "	z
îù€‘	ÿy¿ ÷ =CbqŒÔ™üd5
—u”Ýö�ÞM ñ &¬¸ŠÉ x åu™½á)MŽ-ú
ß·Lµ~Ê	 GÀ)GKc! w²ÜfŠÒö>t´ã"N	Î	ç	’
Ö1:xNV]¿�
 ËQñùBv|+o 4 Š èñé0™ÓYu@	r	ü	@
[
Ä÷¼Y]ÝF  ‘:–€¤AG^C”
ó
_¡·orç(¼3T · ¸�^…+RÐ	¸
WÍ¯	78A¥ä ªÆ_l�Ìà%	õ	
ì
U¤òö <¥ÚÞík˜¨ï>GÎ“	×1û‡Ìèü¹ô`s{ ë &ãBp‹‡ û�Ÿ!	K	–‰.�“L w v±ÛF³¿ðù
uÞJnÉ  ‰ �Åçk@]BX	$	?	û	ò
 ®ö6n@Ü& v ê %;�¤ÄÙæì—oŠEj=~?\ïúŒJ	ø
ÿ
Ö0•-†çÛÈr8 O P u › Ø é CDÙš%&'()*/07M<}£îù'v	./gh+,‚ƒ„…ÀåæØÙÚÇÙëWXeopq Q Y ‚ œ § ² Ð Ù ä —#›þ8;OX¤Ëç(m|
È
i fˆ U EÚ+1Nƒw	I„GKÁ,íÚìY D 4$2Œn¢:
}
`A½g‰  R Z � Ç î Ñøû<DY¥°ÌÒ—
ßêZ{£­x� ƒ � ¨ œËÿ9{§êP×®É
Ú
%32 ³ Ñ Ú å r˜u�–è )Ë¬
dðjH )5ÎºÛ
£Çë3Wx	ª	lû¼f²Û V W,J¯�ÅÏ
ä
®HZ a2O„±	"
oêÂ-“Áí( Ã Ffžg’
J(…I�Lîj W6æ3F	ï	~
„
°
™# 1 ?V„ðÈàZ	†	‰	;
aÚ	&¾Å E Ÿ%¥w\ù�f
ÄÏŠ 5E”¨o£§ž	s
BAOƒh [ È ï ù Òy�Áå	-QE ¶
Jv[|º S â sŽùüCÌ{0=Z›®±ÍÕþö
ù“‘ë0«y  = ‘ y¸Öå8ä¦¨ª¬Ó˜ßQ
"$ 9 ó4½ÿÉ	
ˆ
ç
à™ÛšÁ¤®‘  ) „ {�&<h	Ê
&†4rÔ  "‡®dÈØâ"|Ø7
S
"]´wß0  ? I l “ © ¼ ²ÄÌç:Qa†š¯Ñià ž MT÷ ¨ëo}Œ	Û
¡3s Ò ™r®ºü›Ûáý	eks•~ö#   ´ æ Njsv‘r–ºÞ&JJ‰¸*ÖÜ
­
ÊñbâIä¶Ñ ‘—é½Ì	‹žìXa«ø-6 Û Å:Åh
Ð
èh@–ør'ÈF	 ¾ßé÷6nMy«	b
âî§œ¦„	 ÏÜé
\FŽ½—³ *6aÏÒû�…€¤Èì4X»õl	}	V
ÇgÜ Š»NZ¡ÝôF´y	¢	müoz±Ï‰ ?.]Þså	
>
ì‹¯Òÿ8x[ W 	-K­9MVžÐ@
Ø
â
eI
 ; j ñÆ‚
å
 Ã¸ %LXë(°1ã	à
Œô‚ bj`]	²	#
4¡›¨+;\) ^”õNº�pëö” íP®²¿3‘.Â=   03ak�³×ûC…¥ÒÃ�fî“ ó G“7
)Ö[PÃÕ‹°ý Ä g"óƒ	Ë	›M>¨ïkC	 ~ *ðÂ	KÝ#òT mŸ'h
Š
–
†ÊJ‚Ë$ ~gµ-r	Å	M
ˆ:N -
7¡ÂçüG	p

…
«¹Ö 4yð	±
ªá4U^Õ1‚•^ XÒ’ó	™	l
¦
>û�ä®š	$ 2  çWábp	�
zÛ'NawR  B d@Ï…�É=	¯	Æ
bÜ
Ž 4w›¿ã+O‡	Š	>œ¿Æ+ ¼Ÿñ/>[	j	<
£Z¯6Z  &}¦úw—	0
Â
Ä
?AÓX F † 	ÖµxkŽü
%ÅŒ€ — ® d(¨!´	»
Ì
Ãg6kÐ‹ + ]·~gNàd…û= 6\w•×¨E
(-�«½¹`a„ ¥ R#©(lp¤Óe	Ÿ	2
ZCkºÕ-! ¢F_	b	;ÎC·tP’Ì z ‰+	R	%
*
t

6SÎ{9nB˜i \ É ú úŽeÄK'Ç L ð µalÓzžÂæ
.RÂ‰øõŽD P�s!wM\}
 FÏ·
¶7Xã» ˆ °ÚÍ²¾ˆËIKz" T � š ¦ ± ã $Ž–£Ø‰™ý$D|>[æíøl÷
þ
-”ÿ+ìÆ � q3t�Ê÷úz
1•¯ÖÿÊ9
«
$_ÞG2Y1¬ Â ¹fIœÎ©	ún­é’ Uƒ§ï[åøßÃ%˜"‚ á ·äBË7z¼/š§©«­Ô’!#   ( > H ’ !†zÃÇ×áæ!%…™R
…h/"  M­¹q•¹Ý%9I�·Ú¼à	g
Ï
Éçgr”
áWã `‰„~5mó ³U
è
áíy¦–›¥ : >-êð8ÑówÂ ôMjŽ²ÖúB¾:e<’ ï6‚	Ê	
‰
•
šÕÜ"=§Â¯ü }f´Á¥
‡3Ô0”Õ  A ÿ »žÎ=<	i	o	yÛ=›� * … – ­ Õ|¶ v}Ë
f5jÒú 'Q	',€:jz8`ms‘— K ´Œˆµâ Ž ™ ¥ ° Á #2¯eˆÉÙ#	”åìÉ¨	8
#^ÊþF1XÅ   ' à ¬ÂÆàã T»$.¶ÙÎ
æf“àâ! ˆ,éï}i�±ÕùA75²�	T
”
Ô!x<¦Á9û; @ • ¬ | Íµ|P	eišÓ_“$  J À ³+«ÁÅßèShŒ°Ôø@‹#‡±€	§	ýáÄ:*  	 ” ª « ½ ¾ ¿ yz{Äþÿ*ªÞR´bcde°ìíî/	Û	CDabcd¡ÒüS9 
 m Å;WRf›Àï0	Ü	½
=E£è¢jT?J Ÿ N�X~�	¶	Ð	·ð .&kPêâ  Uôø^Sg$œº	Rïož¢0@ <?ìpÁéØ£4UtßK  b n 	Æ©ð1	Ý	é	¾
Ü
>‘—F¤Å­ Ó šs¯å	Ž	Df°¸ñ!'ëã ƒI»c_l ñYˆÜâ·	Ñ	P´¶+tl‰��÷   O‚ýœþÔ	›§/–Q  ! µ OVs—»ß'K—+×÷»	ó	
Y
ËòxSµJ[å£ý1  ç 9t5Š%�¬ÔsŽã·h¡ò / º õw’â‡	Ýû®
´
ØpŸ?qÏÒ• kÍù_KTh¹óKÖ
2ŸÐcÅðÓ‡A ³	­4b¬VuL K@íq˜ÂÍ¤f-  =\¹’ê¾ê�wÙ;Ÿí7•àù.7
 - ¾êŒY5ð c ª¯´ÆñÞ	ê	Ñ
’PÆâiA¨òG 
2D;ª€UÝ
?éG3~sÛ$(
  ii
¿
˜À—ùçï o Ü IÇ‚†¼ÆSm‘µÙý!E2	¥²ìƒ®É ¿óy¤Ùäêø7ÈÙNæ	�	c
œ
Erò¨�<§ä 6 ›zà	ì	
ï
Ø(ù… t°±o¬	-
B
|±ÆÛã"k…Ë[ªßì Ô Éà¶ÏÐ[óB	×	
G
™
g¹¿ïC×†œ õ „Õ¼›Êd`7	U	]
ê
Â]¾Ç½� s Î AÌê•t	ù	
‚G¾é	 U°p¬_˜´ô ÐÝJ^w
m‰ÈY�‰€ +bÐ2Õi)	(
Ž
Q·ùŠ€ Œ‚—�¥Éí5Y¼Ýö©
ƒ+I Œ £ Óòü†‰m	~	¸	*µ8A¡,PÈmh‘øÝH 7IG€Z•àªãÒ	W
u‡ŽŸ e ¡ -EëÎÞ€5	Õ	 
np6{Å² g ^[¢Â9µz	þ	w
ý}cl( ÷ÿ/;ƒO‹¬¹þõ‚	J
¢
Ó
/:Œ”œ¨­p»Ñþ0]R²ÐŠÿ *8P‹¼í v�ÔGÿ£	Räb— R´ " P@W¿rØ¼	óÿ$Œ�°y\ <T%«‚˜·4
y
íy¹ÓtÝþ¾ Êät˜¼àõ(=L,øÍ	æ	A ¶9K´*U ¶ Ž/]^•˜Òß*tô	

?
Z
VÌT £q\æñõ¤'2E u§FŸAWž	t5m�¸mó X × è :".6Li:NW‚&ÕfFJä×Vdn  Æ 
¦C‹Ñ–­
Ù
@¼é¢ 4`®
ã
k€e±i' 0 “¤Ç¦E	…	�	ƒ
¯
	ÙÄÎ@ < xòãÞµ
æ
õ
Iu/¹  k » LSö`n
;g	!\q rÓ iÄIˆÕü	 
aqª&}EÐ5 ÎúMYèöºôEL|	¡	E�ÆÎˆƒ i $¬LUrä	×
ß
á
ëd· ]“ì`±i23 Ñš§õ* } l!&)Á	OñSÔ#B ‘, 		Ä	L
k
o
=ª9œ©T­]M
 a¢Y®5~v* c´³	º
Á
MÂß>c<W [¤Q"kÒ^	a	d	$
5Yª¼Í_¹, ù`k�OrÃÎ÷ôC
 € p•¢ökè  L…Ap”¸Ü $Hy÷™„»	‘qVg. _=LîòÐìò¥•v	 ºÀ¤
xÚ</– ß 1îº-“ë4Óe’E¥8ú  Q V¯³¿ë.	¦	Ú	<ç ™ ûRÃ8>  €>]�–ï%î�éá/ ðHb‡š¦¯µŒ . ¹ 4†JöÕ
1žÏÓr�´ÄZÒgÎñ”  ~Ž½:6”ï 1…©®»Cl�´Øü DTO±¿ë2‚Ú#§æñ È£°µÎãÇØòß	ë	

,
˜
î
×j„BÖø;©› r Í ô ¯ÉT	ø	

ˆÇXóŒ H‹”(	'
�
*O†H d ),:ŠŸ«ÁÍÓ84	v
Ò
9“5QÐã\QÄ±þk �¾Éãqô<Ì	@U#œ³Üðô)TD Å 3hE�V	j4?°l& hKR„	œ	Ht©%pÍD | \ #«À	Þ
ê™¦NÍ¶ !+ŸÃ	j
À
LX©ÁÌÞ}  o¡jKo“·Ûÿ#GxñjçóU  Þ U-	¥	æîd$˜úè
 }ï„BI™´"ð q ®(‰¢ÀÈâÌ×'	3	÷		
Œ
—
8‡NWƒ…š [JÈgp	²Ì¯Ì% Ý n iƒTn’¶Úþ"FW¨³Ëí p ¬­abcdefno‡ˆ½¾¿ÇÖ‹
TUV¦§KLM„±ÊË  (:  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           	                                                                                                                                                                                                       
            	               	                  '                                                                                                                                                   D   @            
         ?                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  	                                                                           
    	     	      '                                                 D @    
   ?   [�  \  \'  \9  ^Ç  aý  f+/_n]ô :‡ˆ‰ŠÇâãj*Ðç€(£Ø‰ZQA÷è*MuËé.0QoGSºØA	›	ö	
Ÿ
.‚Å7s÷| P
€ÊP
€P
€}ƒ#(»@L 
†
Ï×;aá�±Ÿ—ò’ë,pà .?HÞ	ê	\ac‡tQ«Ò
3 ðÃfÛàÐ™	g	œ § þ8éO/t�ÝN„QBË B ¢Èâb×ÉÈ7
"]0åÉB ™	Ã©¦„ÉQµt[¥f
7 2ýÇ	†
ÙÈ²=Q"Ðvõý·t		H
Šü` ‡œ¨§tÃÉ&MQ¯Ø« Úxr�ñpôÈ`ºô¾pq¢£â+EÍÖ×T	Ã	¨¡m¯Þ	ê	Öi¨»qÇÍ
+
ÕêŸ
ÐK
81
pÐ±Yô‰‚©¦å„ÉQBË / ØE ~Ž†Çò xŒ�êšÍÔ ËD¥	 	›
Ÿ
7TÁ<lóÍ£¥Ã‰”êööTüi3*¢â×ÍýÄPâ„Q Ú/• q^	a	é£ã,cg‹Ó÷?j�	®	B* / Í�×Ž	Þ	ê	DñwÙ;ŽØÛÝ2Æ~ÇÍŽÍ?
V\QþS	 bQ"ŽVbrx�ñ � —ò Š#¯°ëNcÂ 4úÃÚ‰êöÄ?•÷Ç¾q+ŸÃ	j
#œ©, ÈZ£P\”—Ñ)Ñ`	c	X©»^Ÿ¢p¸&Yz²ÉÊ¯þí¢£âãÇ×Ø—
ƒ;š­	_PkðÓA²í\”—Ñ)Ÿ¢p&Z£PÑ`	c	X©»^¸h©%DV5î	ßîŠ&
+9Z£P\”—žÑÏÒ)ZÑA	`	c	Ö	F
u
X©»¾4Ï^Ÿ¢p¸&…j¾q¢£âã+ŸÇ×ØÃ	j
—
#œ©ƒ;šÃHÔ	ÿ	`|4Dr
N›¨\Éãô<Ì	@³)T�uX	í	q
Äq&Eö´ZavZÛÚH�RGbn“«	²	å	â)ÿ8;aö´Zvät˜¼à(L,¶K´i3 š§* Ün°Fß©ž(‰ÀÌ3	8òxØ›
q/|‹	c¤ï{€ÐÝwãÒ	m‡Žš—xÆ'­4òýuî$èÝjî’	úû_¸Ì~H6ò.“
m¬ ;†ÀYâA† 	’ =\�íà.{/¤€Ì~ò.†Y|‹	cï+9žÏÒZA	Ö	F
u
¾4Ï…jät˜¼à(Li,3 š§¶K´*?„Èa	ôožy„Ge ¡ -EëÎÞ€5	Õ	 
np6{Å²d ),:ŠŸ«ÁÍÓ84	v
Ò
9“5QÐã\QÄ±þk5 òxØÏZA	Ö	A
F
›
q{¾ÅÚÊZÞ…ƒld· ¸�^…+RÐ	¸
WÍ¯	78A¥äf öþ.7D]êì¸u�
	ý	I
Ÿ
¡
.o‹¬ºýa|b³ù`k�OrÃÎ÷ôCqûšÄÛ?Š•�ë÷`µÇõ÷,Ë Y{á@IÊÑš	¾	h!3å¯ÿ
È£°µÎãÇØòß	ë	

,
˜
î
×j„BÖø;©›0 “¤Ç¦E	…	�	ƒ
¯
	ÙÄÎ@· Ë Yª¸ÆY{�á^_l�§Ìà@I…ÊÑ+RÐô	%	š	¾	Ø	õ	
m
¸
ì
WhºÍ!3M¯	7<U¤åð8£¯ØòöÿA›¥ä
0 < á x“¤·äòBUƒ§Ëï7[z¼ãåø/š§©«­ÇÔß¦ÞE	…	�	ƒ
¯
µ
æ
õ
	I’ÃÙ!#%u˜ÄÎ"/@‚¹–*6aÏÒû�…€¤Èì4X»õl	}	V
ÇgÜ¥ R#©(lp¤Óe	Ÿ	2
ZCkºÕ-!ƒ � ¨ œËÿ9{§êP×®É
Ú
%32}ï„BI™´"ð…½Xa	8	V	^
x{¿O Ž ™ ¥ ° Á #2¯eˆÉÙ#	”åìÉ¨	8
#^ÊþF1XÅ~ · *6a†¸Ï�Òû^�…¾ð*€¤Èì4Xb…ð+»õRÐ	9	l	}	”	Â	V
¸
KWÍ|¯Ý	(7À#8ÇòTAg¥äÜƒI»c_l  4Ë     ¶  �         �{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’6ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž�¼Ì<ÿÌ¶`Zçç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰º[™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,"<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="26" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2224-2226" color="#a6acb3" type="standard"/></Feature><Feature recentID="25" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2227-2239" color="#ffcc99" type="standard"/></Feature><Feature recentID="24" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="23" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2205-2223" color="#ff0000" type="standard"/></Feature><Feature recentID="22" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="21" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="20" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="19" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3132-3720" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4682" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="3891-4682" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4683-4751" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="2778-2784" color="#ffffff" type="standard"/><Segment range="2785-2802" color="#ffffff" type="standard"/><Segment name="-35" range="2803-2808" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2823-2844" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2730-2746" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2754-2770" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1812-1933" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1375-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1728-1759" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>1772863c-6a01-4be8-ac68-e566cf24ddda</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="0:53:2">2021.4.16</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..4750" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..4750" colors="black"/></BottomStrand></StrandColors>  ^X     
#  'c        'c{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’6ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž�½‡Ë¥ÑÕAUCHªgQ‡ÕD+«Q3„æ¥[Ãšö¶©Ð¾<0CUû©3:9US/‰c†7mÄíuÕ–ƒ§�ÄÕT–ÇÒr²¸¹•Tat!!WƒAƒÆŽ…8•Vg8jqÅ©ì®SãYfêæÆiPÈãÎ&•X|oª#26üjó-ºÝMoNcÃ&aœÔc:ìt)iö*hi•±ÑÓ¥ÁprEaLÍÖ‰s²M\ŒWÎàGtÓ¤øÀë:a³Ÿè÷4Ôv¾l`RQªÕÊblf¨Ûo:}X¨QV5TÕ<U[j‘E‰E™áEQèUã]Z¤º¥MrÄ�ü†ÕÅt&¦j¤ÏkWTqE¨3È‘AuS…vªÎuG0æ	4áIñ…Z›o–ÏU©¥�µ\ÒM1¦Qqœ)¯[N &µmTQÉT®¨~¦óJ…SŠ½1µAU¡K
•IhU„URWo…èEI´ÛÉTmÓÂša2k^�ƒµQŠž¿�UµŠ®ó¡E4ÁM5SV×Æ:¥s.®–—ÄQ…1J†RVÍ…^GTñªÚtÂúŽ™jˆ]¬ùT‘DTßƒ°^»ií]Or„oCøŽ7S²?ö×à²l1#AD<u4âqøýöôÑNÓ�b±8FÿSëÐUD^8aÄlõ0ÌF„ÒEáýÁaMsDV…Íuj’ê•44ËUþòZxš™Â¥¯]TSM=‘<«Û¦P
‡]­âZ2¡´!Ã‰*å8áßšÑU!­EU�4„àãAU6­¶ÜW	<ê'Zœm¯iV¨ßjeBT¸ÐÓš¥ÕFDZÞã­AXQ X…Zž!†tN•TÓÅ…L…LU]Ei-é·ÐQÒª…QUYNQi­Õ]R"FvNU›:˜{ŠUE MQÉx3NF�tÈ«®ü»�yJÉ\VE­5C¡VT8¨RØ<þm«±:ŒÈëui#^»ií]Or„oCø„øSDØelþ¾Ç!¾Pm@Çá¶îlðÈ‚'àn#†ïL�_à�T@×ÝOÛ}14¬u4TA8`sßOÓRVû€Óß‚L3ÜZ«(¡MN b…@ø�?"ý\4[ß�¿CÅÐÓ²|†ÄãŒ4l�pÜöD�CþMnlþ_�DQ�ÄáQ¬<Aø]±øîOŽe4×V©.©SCA±_à òÝv4M7Û r8Fø@Ë!³À·H³Ç ^8}µÃ†È=2PõEl6‚ùøM5=uÔ;�Duãq0áWxEÕ3™Ïh3^»ií]Or„oCø„ßôëMõ]þž²=5ÔÜ:“ìvÓár òL7Øm3ÕzÇŸ„Äv±D1H?Cø¸M*Oƒ“ûÄ¹`Å<C÷­½øQwÔnxÃ5ôÔFëƒ0Ôlñëà[LsÔ½ôÔRôí­\{÷ªÊ(SS€¡D_Ð>!‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²~‡ç�#¬üçü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×Ou™·ÏM\6Ô!zÔ=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô”Zçç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰º[™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6  ¿  
i  
¦  ¢  ó    A  y  5  F  ¬  ½  •  ¦   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1375-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7144-7265" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8086-8102" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8062-8078" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8155-8176" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8110-8116" color="#ffffff" type="standard"/><Segment range="8117-8134" color="#ffffff" type="standard"/><Segment name="-35" range="8135-8140" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10014" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9223-10014" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10015-10083" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8464-9052" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6365" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="6312-6368" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5876" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="5823-5879" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5501" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="5448-5504" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5144" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="5091-5147" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4784" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="4731-4787" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3899" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="3846-3902" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3560" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="3507-3563" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2720" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="2667-2723" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="3567-3827" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="4791-5072" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="5151-5429" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="5508-5804" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="5883-6293" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="1728-2651" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="2727-3491" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="3906-4712" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6372-6374" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6375-6377" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6378-7091" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7537-7555" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7559-7571" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7556-7558" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="7:2:36">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6370" colors="black"/><ColorRange range="7091..10082" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6370" colors="black"/><ColorRange range="7091..10082" colors="black"/></BottomStrand></StrandColors>  ^X     
"  '_        '_{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž�½‡Ë¥ÑÕAUCHªgQ‡ÕD+«Q3„æ¥[Ãšö¶©Ð¾<0CUû©3:9US/‰c†7mÄíuÕ–ƒ§�ÄÕT–ÇÒr²¸¹•Tat!!WƒAƒÆŽ…8•Vg8jqÅ©ì®SãYfêæÆiPÈãÎ&•X|oª#26üjó-ºÝMoNcÃ&aœÔc:ìt)iö*hi•±ÑÓ¥ÁprEaLÍÖ‰s²M\ŒWÎàGtÓ¤øÀë:a³Ÿè÷4Ôv¾l`RQªÕÊblf¨Ûo:}X¨QV5TÕ<U[j‘E‰E™áEQèUã]Z¤º¥MrÄ�ü†ÕÅt&¦j¤ÏkWTqE¨3È‘AuS…vªÎuG0æ	4áIñ…Z›o–ÏU©¥�µ\ÒM1¦Qqœ)¯[N &µmTQÉT®¨~¦óJ…SŠ½1µAU¡K
•IhU„URWo…èEI´ÛÉTmÓÂša2k^�ƒµQŠž¿�UµŠ®ó¡E4ÁM5SV×Æ:¥s.®–—ÄQ…1J†RVÍ…^GTñªÚtÂúŽ™jˆ]¬ùT‘DTßƒ°^»ií]Or„oCøŽ7S²?ö×à²l1#AD<u4âqøýöôÑNÓ�b±8FÿSëÐUD^8aÄlõ0ÌF„ÒEáýÁaMsDV…Íuj’ê•44ËUþòZxš™Â¥¯]TSM=‘<«Û¦P
‡]­âZ2¡´!Ã‰*å8áßšÑU!­EU�4„àãAU6­¶ÜW	<ê'Zœm¯iV¨ßjeBT¸ÐÓš¥ÕFDZÞã­AXQ X…Zž!†tN•TÓÅ…L…LU]Ei-é·ÐQÒª…QUYNQi­Õ]R"FvNU›:˜{ŠUE MQÉx3NF�tÈ«®ü»�yJÉ\VE­5C¡VT8¨RØ<þm«±:ŒÈëui#^»ií]Or„oCø„øSDØelþ¾Ç!¾Pm@Çá¶îlðÈ‚'àn#†ïL�_à�T@×ÝOÛ}14¬u4TA8`sßOÓRVû€Óß‚L3ÜZ«(¡MN b…@ø�?"ý\4[ß�¿CÅÐÓ²|†ÄãŒ4l�pÜöD�CþMnlþ_�DQ�ÄáQ¬<Aø]±øîOŽe4×V©.©SCA±_à òÝv4M7Û r8Fø@Ë!³À·H³Ç ^8}µÃ†È=2PõEl6‚ùøM5=uÔ;�Duãq0áWxEÕ3™Ïh3^»ií]Or„oCø„ßôëMõ]þž²=5ÔÜ:“ìvÓár òL7Øm3ÕzÇŸ„Äv±D1H?Cø¸M*Oƒ“ûÄ¹`Å<C÷­½øQwÔnxÃ5ôÔFëƒ0Ôlñëà[LsÔ½ôÔRôí­\{÷ªÊ(SS€¡D_Ð>!‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²~‡ç�#¬üçü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×Ou™·ÏM\6Ô!zÔ=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô”Zçç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰º[™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6  »  
e  
¢  ž  ï     =  u  1  B  ¨  ¹  ‘  ¢   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1376-1394" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1412-1430" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1466" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1484-1502" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1520-1538" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1556-1574" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1592-1610" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1371-1717" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7140-7261" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8082-8098" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8058-8074" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8151-8172" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8106-8112" color="#ffffff" type="standard"/><Segment range="8113-8130" color="#ffffff" type="standard"/><Segment name="-35" range="8131-8136" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10010" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9219-10010" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10011-10079" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8460-9048" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6361" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="6308-6364" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5872" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="5819-5875" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5497" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="5444-5500" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5140" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="5087-5143" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4780" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="4727-4783" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3895" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="3842-3898" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3556" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="3503-3559" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2716" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="2663-2719" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="3563-3823" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="4787-5068" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="5147-5425" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="5504-5800" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="5879-6289" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="1724-2647" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="2723-3487" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="3902-4708" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6368-6370" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6371-6373" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6374-7087" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7533-7551" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7555-7567" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7552-7554" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:40:54">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6366" colors="black"/><ColorRange range="7087..10078" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6366" colors="black"/><ColorRange range="7087..10078" colors="black"/></BottomStrand></StrandColors>  ^W     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÏá…øENŠÁàsÄ…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:39:52">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  _9     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÏá…øENŠÁàsÄ…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   ˆ       ®ZTR

BASE       i  xÁA  ƒ°Yãx` þµ,9ImM¥,	¦èŠ©1)¤Â•JÎm1#—Ë’˜­ÀSA´‚²D]hIÌæ*˜©‹º‚b…‘ˆZ›…+L£Ð2CW©s•¤ËµxªˆMk   ƒ<AlignableSequences trimStringency="Medium"><Sequence name="GVPK" ID="0" sortOrder="1" trimmedRange="0..281"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:39:0">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  _:     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÏá…øENŠÁàs„…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   ˆ       ®ZTR

BASE       i  xÁA  ƒ°Yãx` þµ,9ImM¥,	¦èŠ©1)¤Â•JÎm1#—Ë’˜­ÀSA´‚²D]hIÌæ*˜©‹º‚b…‘ˆZ›…+L£Ð2CW©s•¤ËµxªˆMk   ƒ<AlignableSequences trimStringency="Medium"><Sequence name="GVPK" ID="0" sortOrder="1" trimmedRange="0..281"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:38:55">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  _:     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÏá…øENŠÁàs„…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   ˆ       ®ZTR

BASE       i  xÁA  ƒ°Yãx` þµ,9ImM¥,	¦èŠ©1)¤Â•JÎm1#—Ë’˜­ÀSA´‚²D]hIÌæ*˜©‹º‚b…‘ˆZ›…+L£Ð2CW©s•¤ËµxªˆMk   ƒ<AlignableSequences trimStringency="Medium"><Sequence name="GVPK" ID="0" sortOrder="1" trimmedRange="0..281"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:38:49">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  _:     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÎá…øENŠÁàs„…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   ˆ       ®ZTR

BASE       i  xÁA  ƒ°Yãx` þµ,9ImM¥,	¦èŠ©1)¤Â•JÎm1#—Ë’˜­ÀSA´‚²D]hIÌæ*˜©‹º‚b…‘ˆZ›…+L£Ð2CW©s•¤ËµxªˆMk   ƒ<AlignableSequences trimStringency="Medium"><Sequence name="GVPK" ID="0" sortOrder="1" trimmedRange="0..281"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:38:42">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  /      ´  …         …{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èÀüÃÏðËf®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &X<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3122-3710" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4672" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="3881-4672" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4673-4741" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="2768-2774" color="#ffffff" type="standard"/><Segment range="2775-2792" color="#ffffff" type="standard"/><Segment name="-35" range="2793-2798" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2813-2834" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2720-2736" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2744-2760" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1802-1923" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1712-1755" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:11:18">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..4740" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..4740" colors="black"/></BottomStrand></StrandColors>  /z     .  o         o{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èÀüÃÏðËf®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &X<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3612-4200" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5162" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4371-5162" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5163-5231" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3258-3264" color="#ffffff" type="standard"/><Segment range="3265-3282" color="#ffffff" type="standard"/><Segment name="-35" range="3283-3288" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3303-3324" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3210-3226" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3234-3250" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1802-1923" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1712-1755" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:10:30">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5230" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5230" colors="black"/></BottomStrand></StrandColors>  0     M  é         é{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èÀüÃÏðËf®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &i<?xml version="1.0"?><Features recycledIDs="1" nextValidID="20"><Feature recentID="18" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1712-1755" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1802-1923" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3356-3372" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3332-3348" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3425-3446" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3380-3386" color="#ffffff" type="standard"/><Segment range="3387-3404" color="#ffffff" type="standard"/><Segment name="-35" range="3405-3410" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5284" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4493-5284" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5285-5353" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3734-4322" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:1:6">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..1363" colors="black"/><ColorRange range="1923..5352" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1363" colors="black"/><ColorRange range="1923..5352" colors="black"/></BottomStrand></StrandColors>  0Ê     P  ÷         ÷{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’§¿›HdEÉ…§¿›HdEÉ�§¿›HdEÉ”§¿›HdEÉ‡Ò§¿›HdEÉ¹Ò§¿›HdEÉ›Ò§¿›HdEÉ‹�Èœ 3æU4NÊ’ËFÏ�5¯uÞ¨ºg¡ûÙk‘91k¥lÌ<ç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºW¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ')<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1816-1937" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1731-1773" color="#ffff99" type="standard"/></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3386" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3346-3362" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3439-3460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3394-3400" color="#ffffff" type="standard"/><Segment range="3401-3418" color="#ffffff" type="standard"/><Segment name="-35" range="3419-3424" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1589-1607" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1553-1571" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1517-1535" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1481-1499" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1445-1463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1409-1427" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1373-1391" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1714" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5298" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4507-5298" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5299-5367" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3748-4336" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1938-2059" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:0:25">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1937..5366" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1937..5366" colors="black"/></BottomStrand></StrandColors>  0Ç     L  ç         ç{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’§¿›HdEÉ…§¿›HdEÉ�§¿›HdEÉ”§¿›HdEÉ‡Ò§¿›HdEÉ¹Ò§¿›HdEÉ›Ò§¿›HdEÉ‹�Èœ 3æU4NÊ’ËFÏ�5¯uÞ¨ºg«‘91k¥lÌ<ç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºW¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ')<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1800-1921" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1715-1757" color="#ffff99" type="standard"/></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3354-3370" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3330-3346" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3423-3444" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3378-3384" color="#ffffff" type="standard"/><Segment range="3385-3402" color="#ffffff" type="standard"/><Segment name="-35" range="3403-3408" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1589-1607" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1553-1571" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1517-1535" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1481-1499" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1445-1463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1409-1427" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1373-1391" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1714" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5282" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4491-5282" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5283-5351" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3732-4320" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1922-2043" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:58:39">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1921..5350" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1921..5350" colors="black"/></BottomStrand></StrandColors>  /i     #  C         C{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’§¿›HdEÉ…§¿›HdEÉ�§¿›HdEÉ”§¿›HdEÉ‡Ò§¿›HdEÉ¹Ò§¿›HdEÉ›Ò§¿›HdEÉ‹�Èœ 3æU4NÊ’ËFÏ�5¯uÞ¨ºg«‘91k¥lÌ<×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %ô<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1715-1757" color="#ffff99" type="standard"/></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3190-3206" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3166-3182" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3259-3280" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3214-3220" color="#ffffff" type="standard"/><Segment range="3221-3238" color="#ffffff" type="standard"/><Segment name="-35" range="3239-3244" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1589-1607" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1553-1571" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1517-1535" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1481-1499" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1445-1463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1409-1427" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1373-1391" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1714" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5118" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4327-5118" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5119-5187" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3568-4156" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1758-1879" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:58:14">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1757..5186" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1757..5186" colors="black"/></BottomStrand></StrandColors>  .P     !  ;         ;{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’§¿›HdEÉ…§¿›HdEÉ�§¿›HdEÉ”§¿›HdEÉ‡Ò§¿›HdEÉ¹Ò§¿›HdEÉ›Ò§¿›HdEÉ‹�Èœ 3æU4NÊ’ËFÏ�5¯uÞ¨ºg Ÿ˜ØòS­"—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3182-3198" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-3174" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3251-3272" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3206-3212" color="#ffffff" type="standard"/><Segment range="3213-3230" color="#ffffff" type="standard"/><Segment name="-35" range="3231-3236" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1589-1607" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1553-1571" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1517-1535" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1481-1499" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1445-1463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1409-1427" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1373-1391" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1714" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5110" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4319-5110" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5111-5179" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3560-4148" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1750-1871" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:57:33">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5178" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5178" colors="black"/></BottomStrand></StrandColors>  .R     #  B         B{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~c`oÉN´Š^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3189-3205" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3165-3181" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3258-3279" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3213-3219" color="#ffffff" type="standard"/><Segment range="3220-3237" color="#ffffff" type="standard"/><Segment name="-35" range="3238-3243" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5117" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4326-5117" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5118-5186" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3567-4155" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1757-1878" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:55:54">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5185" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5185" colors="black"/></BottomStrand></StrandColors>  .¾   
  #  C         C{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~c`òS­"—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1758-1879" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3568-4156" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5118" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4327-5118" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5119-5187" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3214-3220" color="#ffffff" type="standard"/><Segment range="3221-3238" color="#ffffff" type="standard"/><Segment name="-35" range="3239-3244" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3259-3280" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3166-3182" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3190-3206" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:49:31">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5186" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5186" colors="black"/></BottomStrand></StrandColors>  .¿   	  $  H         H{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~ccßü”ëH¥è©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.¡íXbÖ’æÑNn¿¯ Goâ¥š•ÓH¯Õà+¦oèî_B�¯‹íÐ\RþTWH4ô*OðZ¾ª�?h&ªÿ&ÿô"ãCµDÓL­TÆü�~¿Èü/ós�ÀÀ3ÁðLü;8ãÿ“ –�¿€  ‹øfæ]EY™™e)·%$]…eÖe¦É„¥n•KrUmŒ¶¨{s)’µ[Hw§Í¡ÔÜûp½L1á¾V7Lpk3š‘Q5šµ62Ö©Ín®@¥¹8ÛA¶l°.ª¼;KlýNæà6ÀQOÈú¯ÌB…0Ø£Äxã�¡Êsr§Øa¬ƒØÜú—^++Cß€gÒ²Ì:¯t¯OÅ3m5ü]gÃ<�#Â#F–0Œ3€fœ´Ä	¾<ÑX�ŸòŸÃ3 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1763-1884" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3573-4161" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5123" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4332-5123" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5124-5192" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3219-3225" color="#ffffff" type="standard"/><Segment range="3226-3243" color="#ffffff" type="standard"/><Segment name="-35" range="3244-3249" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3264-3285" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3171-3187" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3195-3211" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:49:28">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5191" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5191" colors="black"/></BottomStrand></StrandColors>  .Á     &  O         O{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~ccßÂ<òS­"—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1770-1891" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3580-4168" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5130" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4339-5130" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5131-5199" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3226-3232" color="#ffffff" type="standard"/><Segment range="3233-3250" color="#ffffff" type="standard"/><Segment name="-35" range="3251-3256" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3271-3292" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3178-3194" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3202-3218" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:49:18">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5198" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5198" colors="black"/></BottomStrand></StrandColors>  .¾     #  B         B{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚ðoÉN´Š^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1757-1878" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3567-4155" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5117" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4326-5117" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5118-5186" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3213-3219" color="#ffffff" type="standard"/><Segment range="3220-3237" color="#ffffff" type="standard"/><Segment name="-35" range="3238-3243" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3258-3279" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3165-3181" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3189-3205" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:48:50">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5185" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5185" colors="black"/></BottomStrand></StrandColors>  .¼     !  <         <{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚ðoËH¥è©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.¡íXbÖ’æÑNn¿¯ Goâ¥š•ÓH¯Õà+¦oèî_B�¯‹íÐ\RþTWH4ô*OðZ¾ª�?h&ªÿ&ÿô"ãCµDÓL­TÆü�~¿Èü/ós�ÀÀ3ÁðLü;8ãÿ“ –�¿€  ‹øfæ]EY™™e)·%$]…eÖe¦É„¥n•KrUmŒ¶¨{s)’µ[Hw§Í¡ÔÜûp½L1á¾V7Lpk3š‘Q5šµ62Ö©Ín®@¥¹8ÛA¶l°.ª¼;KlýNæà6ÀQOÈú¯ÌB…0Ø£Äxã�¡Êsr§Øa¬ƒØÜú—^++Cß€gÒ²Ì:¯t¯OÅ3m5ü]gÃ<�#Â#F–0Œ3€fœ´Ä	¾<ÑX�ŸòŸÃ3 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3183-3199" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3159-3175" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3252-3273" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3207-3213" color="#ffffff" type="standard"/><Segment range="3214-3231" color="#ffffff" type="standard"/><Segment name="-35" range="3232-3237" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5111" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4320-5111" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5112-5180" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3561-4149" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1751-1872" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:45:28">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5179" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5179" colors="black"/></BottomStrand></StrandColors>  .»        6         6{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚ü´Š^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3177-3193" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3169" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3246-3267" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3201-3207" color="#ffffff" type="standard"/><Segment range="3208-3225" color="#ffffff" type="standard"/><Segment name="-35" range="3226-3231" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5105" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4314-5105" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5106-5174" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3555-4143" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1745-1866" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:45:16">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5173" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5173" colors="black"/></BottomStrand></StrandColors>  %[     &  N         N{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚|<ÔŒlïü´Š^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Ó<?xml version="1.0"?><Features recycledIDs="0,4,7,8,10" nextValidID="18"><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5129" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4338-5129" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5130-5198" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3579-4167" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1769-1890" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:41:16">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="1364..1726" colors="red"/></TopStrand><BottomStrand><ColorRange range="1364..1726" colors="red"/></BottomStrand></StrandColors>  1Ì     $  G         G{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚|<ÔŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &G<?xml version="1.0"?><Features recycledIDs="10" nextValidID="18"><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7170" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6379-7170" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7171-7239" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3702" maxFusedRunOn="3702"><Segment range="2317-2979" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3103-3702" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5620-6208" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1766-1977" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3810-3931" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3099" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3702" maxFusedRunOn="3702" detectionMode="exactProteinMatch"><Segment range="3049-3102" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ª<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:41:7">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="1364..1726" colors="red"/></TopStrand><BottomStrand><ColorRange range="1364..1726" colors="red"/></BottomStrand></StrandColors>  1Í     %  I         I{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚|ÃÍA¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &G<?xml version="1.0"?><Features recycledIDs="10" nextValidID="18"><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7172" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6381-7172" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7173-7241" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3704" maxFusedRunOn="3704"><Segment range="2319-2981" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3105-3704" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5622-6210" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1768-1979" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3812-3933" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3101" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3704" maxFusedRunOn="3704" detectionMode="exactProteinMatch"><Segment range="3051-3104" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ª<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:39:7">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="1364..1726" colors="red"/></TopStrand><BottomStrand><ColorRange range="1364..1726" colors="red"/></BottomStrand></StrandColors>  1Ñ     (  V         V{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~ccß0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &G<?xml version="1.0"?><Features recycledIDs="10" nextValidID="18"><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7185" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6394-7185" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7186-7254" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3717" maxFusedRunOn="3717"><Segment range="2332-2994" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3118-3717" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5635-6223" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1781-1992" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3825-3946" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3114" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3717" maxFusedRunOn="3717" detectionMode="exactProteinMatch"><Segment range="3064-3117" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:38:56">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="1364..1739" colors="red"/></TopStrand><BottomStrand><ColorRange range="1364..1739" colors="red"/></BottomStrand></StrandColors>   é      )  Z         Z{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬Z•°o0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1710-1752" color="#ffff99" type="standard"/></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7189" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6398-7189" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7190-7258" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3721" maxFusedRunOn="3721"><Segment range="2336-2998" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3122-3721" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5639-6227" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1785-1996" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3118" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3721" maxFusedRunOn="3721" detectionMode="exactProteinMatch"><Segment range="3068-3121" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:38:25">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Dý7zXZ  æÖ´F !   t/å£ã“§] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷¿Üµ¡&õý{Îb¯Ác�õÚÝR¦êg�ÜŠ‹‚/ŒG€ö×Ô¬úÑ¢¼Ñ1 .‡ŠÂ|’ÏÌÙ=‡ÓB‘À¢²‹vøfOàu‹õ9ÚD^‘S÷%G¯&Åû¨ö¯aº8JŸ$(0Ç~Zfe« 8³([– âµ�´èp}%àÉi«Ô3ÜØéë.Üó]]¸ª4¬ *NF¢‡…¨Ï—“¿?1ÂZUÀ8Í‘ÃUs1H�q½±5¨¡oÉ‰�Ù½ #šßé‰€y‰L oÑÅ÷HŸ‰ë«*n"º<ëOK~i‚�‡aí¼ÄÛg"güD{™ µ>:¶£@uÉloÀàÚ4ˆ^Î1{^bµ;.œDÆæÜp 5ä†í†þþôGW×(~—·cäã¬×½A‚ëxuTÚd©Ü´Ó²¾{n—c*ÿøª¥"°¦0IaŒ„­f¤ÛÞÉ/�ÏŒXãŠØ&ÒÉ†w 0Ú+Þ¹–xm¸p"ÃWUœ5Ô¤-¢½-°5ÀNêf]NW‹zDçwÉ Ý4õ�áD±cÕqî_¹2p²±þ_óÊ¹èù‡½vNZÙ½�…<Iék«™Í8úCùß.b3šò§SJØë X»/šÅÜëÁVÞ?r:i.8ÂŠ•€5ú ö�¶ùºÅ¤§ßÎmï…ø¢ÐÛ‚Öc ƒ•D\8™ÆªbŒ¤®ÉòžSoÏe­‰]æƒ^QõV#¨v&Ì*!Q	VŒ0
är¾‡–¼�èèê<ø¬×àëé¾lÝi#u‹Êp/¬}HŒ“WÔèúè‡ü
ei’
ÄQV>€îjþîÍS@Ü7Õƒ&©ßi,y
Üé²oš®GK^ �‹´�†Ï„�˜Íj
mR?Ú3˜ÜÕ“ä} :æ�¤w­¿.¶«TûõH•NÚô°'a~ù
eÎæ1©¿L^ÍÏ;²²úâH¦ÄkmD&1¢’[ÉW�Ædm×Þ»'¥ðã?cj¸VèÉÔÉÞÀM ›eÿß
wÉyåúŽ�úO·.?É]uR�ŸŸH¥ÁGFVÚß#¼ÒóñÉˆ“Lê²…H¸ûÈ1r³¼³°ü0Å©ÂýiÿõJ#ºêy·ô½ú'FHŠsX}‘®t7bÄËgñ ö¹ÓKl	�]dúbgç&HcÝ—"ý;ÎŠ†2˜Ó	4tÓ‹,×–Y þÔ‘ã×¸ÚêXe…rêƒ3ÈLjnUÔÇ:GË÷~Dª|ˆÜ€|íõºÆëB�Ÿ(ÏU<rÑÉ}¯_á/$•×õ'hê$ÄÞ‰úœ{Äk�øRã—BšÜBŒ>mÕÕr;þÓ�Àu2ø�Ù0D9›ö.v{!¾§Hé¯þ¯,Û-§˜½o´¦k‰zT9.3xÏ”ÊåWÞhc	`÷å5ªâÑrÑº�yWý+ã”òÌ5A5¿b<`þàï¡Ìä ÒÄNw™®´$ÏEª¯"|Û^ ‰1nµÕZfèG4¿;F”Ÿ}¼‰—6þŒ %mC«‡bG«Ýä<qþÛ°-€:6¡³t°"OÆžwÃqþ�y´ÌN�ÆÒ=¨‚C‘•_ð™±`kø¢òêI±W%×ŸUE¨Å»˜Ñ×Þ�'À©)ä}›Ùnõøz÷"?»§põ	úOàÏ:öíYM& ¯Ž7†¥BÏ+’p;ÏÉÍÇ›2	 Rï¥©þyËÐ0Ø™Ké7W_ŒNÉR±tÃECŸÌ`‰£éáVo…KO·iuHú½cEô ž©¹}œ¸Ìe—7·»9ŒN×"—Â[ÕEçdHCZö%Î~àÍ Ì¬^ÿíüØ«æ|N˜Yò{ÍçàâmyF,®Ô®öÃÈ†•§ªqLè$ê¼9Âc€þ‚ºÌ¶«vªƒD&Hmã.EŒ!‚åŸKän»XØ™OI�hVa2ç+üwld“–]Ýw1¨y=×ƒe�¬�òÔâ¯Î²ÕKˆ"r9aÑÄncf34_As vÿ;[�U"ÞÐE™"Òw[ÀØ°ÍéH·Re|=[ž•ç�®Ç–À¿o|n¢rž0JUôánÜ˜û+­#`4AÑ”tÏÏ4ÔÛ•Lòóã}ÂªR˜’ÖÐáYáhè0ã72|ç0%“\ûã<ÿHRì [ g@Ü6_)R�|Þ¥•5ÆÓÚ®mâ|—E¶×¢=×Ë
›SzÒºýL7ÞEƒÅåë>&ýªÖHßÆÖünW«¡¥„õ{
êM‘K©Ué‡ÕÑ×õ×hJÿn`òª©ìâ�d—­zª&#tñZ-qêù�ë@�C´¦üì©VŸÞ’'#î‘³‘¨5sÊÓmÅ�:”T<©I�êhãŽ±èâ5@u¬UhÚ÷IŒ�ƒõÎ(iÐ‘‚öåé»>•ÈçeGs[R}3Š0GÅ0&ÃÜ¡™a�é'ò®«…m«ù»ó‚J/Ô‡ÓYÜJòaŠ@±tÞßTü?jˆäÈÿx5Â¾à"ÎÚj©°ˆ¥2Ê6Pvi#ËÏT¡	ÎP§—îdp	¹�ÿv¾>àÔJ�¶étü6Æ‚<YpN[2ÅnÆÞz©w“ÂD3/xšoå»–$Ô?-1‹W»¸¹4¨vÈÆä³æ¯8‹wø‹¶}·mápÅ·³�QïÿTÿÁ47„<4	¡Þ„¦¢BžbPÝ]6Ñ'	2C’ñ ¥@4R¸|#¥G‡ttâ%’Ð^7‘xÁ�ú£=åçK}»ë;A¤®e 
ùIàßNVwõ2Ú2
uF¾šàrgZÎŸ.ì�vH×ÏÀ½˜/òµ\½ÿ:TŒrQ##Ù°É<),hœÁ•z{QˆËú›r8@'œ
mU³aÑþ;BÌ ‚Ê‘Ý]ÆÒQ9Á�ÊÉR•ò>ö1ä™T«Ÿ­"¼)¿rOú¬H4ÒØ¦{3˜mšzoeàaµ¾ƒGúèµŸ9ØSSÏl;/5�®�)]ÿî`žÓ‡ýêÆ‰4È�$q‹ ÙŠ—Ó¢¹Ì cÙ.9È3myÒ.<¦C fÐÄÞ›–+ýÅ¯0†o‚›9J¬Û©<Ÿ‘}¥¤Ð’ÔxF©–~›[†xAŠŒî×Ðty ð’\Ü%9Ô„Ã’`PÆü�ê\­Ü1Å}}/ª_)¶áV)¡ëÊ]÷Éq[œ|B¶ž;Gº öGS)|EÞàízÛ§Ð�»" EçHÓÁÚ?Þ® _…µøÔŠÄ¯›²�·X x7Ãwçd™�µ‘ˆ�•ËL´„¸äïrP«Þ‹Eä[5Q´¤ èK)ÐOÔ�±Ðüg"Ë§ÛAPá›A¡Pìo|:Cy8!29Ižt~ã+—½‘.—£<�¸á<.é%¹©ß]÷·EA?rs¾ä"Ö†mpŒ��’ÿÊŒ‡žÛK™­?�uðátÜWÇhÈà¸CçÎ¶x*aYa–B¥¶7IJËQsá¿×âÛˆ Ÿ%’Kâ3Ne”“¾àÕoåm0•†Îé÷®TCkä­þ|ÈB˜"ðD¥Éc§		_¡ëäÙâg#I×jÇ]ÌÝ”¿mõˆ£¢DR·©�ÎVãß©ŸÆ�*àp™þ²»ˆ×º,¬G¡¤�‰û7×ìŽÍh%ä�zëƒS"ÿÿñKâB€êößÏ6”,ªÁ„Q WE,@pí¾›vž$Å,øFÔÚTtûÖœ hbnîÃ�áríUzL€2·•ö‰W‘Ú\áhÅ³®_‰u¯NSžô„m•#ìUñî²ƒq�Ö¿p_D_ä±Ôj´ˆT>nÙ|ñÏú^-·|SqtuTçœâöIéø¬«F|Au•×&¥Ö—Mç{zŠÒÈ>ËLRŠ¸o|›EDoŸÅ*œ·Çˆ¹!QDk%î›Èuµ˜jP”�h•	ÅÃº�²Ü�hlêêÉj$A·ÆEp|æ»a‚mBÍ$u†2(ï˜ƒö¶Èö0Œ"�µ›‹ø¨ÇØã±¢ƒBQzÅA°Ø¥@¸ºß—>¸N£ô<@Ç �Bg:ößz�hDÛöãqØSÒ=>„#ûÝs˜«ªÈ&¶»‹�ƒ»X¹«™éT¹	Ý¿ŽZ{\{Z7„jFfåAT*~î÷³*A•s¬ø…�x-ëZÝm"€¼…ißô¾“):pÙ”±ó=J1óCêÛ5ìæ6tYƒGØz{Í),CIP&¯?ÄìD£)¬¶Xûÿbûœ#R-§üCÒ^\¯uÓÉqñ	‡ºo )xÉb›–`Â¶–Õt²€cå¦Œ/³Ì +ÆxŒ¹èÅ�Š»¦ëÚ*@Õý\×#ÓÄüÃÞ’lŒ$�d
»\´–*mˆ~€NÄÎ›£ÝŒ1a *»Újté1ý¶z9�Óò\xkñ7[…òùR(BKJJ�én›?òrÒ÷§¥mÁ…Ë¦&›ŒÃ½Õ<Šü.Vƒ‘j�`�V5Ìi££/XÐóåûÕ"EJÌíºm]ôéÖ}É<m�©œ÷ï>Ñù:'´ZQ÷“eBu"±æ\štV¾¶«y8ˆv‚|<*�h¥ÜÆùõTÇÈöˆoÔ:ç7Ü�©ˆÍ²ì*Ä.†Á UâäSÙ.tAnZ~œXSiÁ îïÏ—ùº�PŸYPœD> ìð®7\•¸«˜WŒ™›á²›6HCÁ*¶~Â~ˆHjÏÞÜ‹š(:‰|æQ¦’ÝàÆÓ—WŠêØkEu9°f ‚¿˜Ôkh†ÓX˜¶Ç^ä5ßt‚æ’Ø-BS¥o¦¹ ýq	g¼_K† NÒÌ¶ûïý_%rUÜmÖ	ï¿óª¾jº“ ¢2ÏsÑú†’…ø³±Á®á*ŸÎs>3Õí*}AWßà³ÒA»ÕÙvûYµáÜu°{•Kê³nYJ6Õ·%æ}lE
h8`Ú É[�˜ÀªXÇ¡F½¶×t–�+e¥k”<•ŽÛnÎº˜ø
‡óñ0ÐV
Ø˜’ÐÇÅC¤.+’”à!Êy—séZ½wA)&°�"
šòT�~Ü˜º’>Ç’±Ø¥©,Xj‚M$cNI%“RÄg·ïTB�šîj!Â.glt”¿*2~»rÄéO“ô×ŒÚúÔGžBÌÆNW¸Ê÷ ô)þ›‹<ÒªYúáx±Â4�t˜5\P‡LªE‹Œ;‡†ÐÃT+¾1±¥KvqŒy99œ1ÉøW!×•œ^Îh–Ã>(µYE�}]/�åEõ(S½¦ê3ûdÞˆDã!•N¶Î<9ˆj54{¡ŒÍÇšpßwž¶:6VÖŒy�†X•.
»_'ß4Ù}ŸË†¯w×©@þb!úL44{~!Ü6ÉÔœ]è"ý#¡;ÁØ-Lå+bW¹r8Â¹h©üÏ=¨¾W<eSzçdQ? w®V`£JçÎÃ×³øJ¨§åJ‹xÒl�¾·Ò÷u8ÖzoÇ«w^àRb½�ÖCÁçŒ¥tùwÙ#¸gº'¢êZRªþ5à+Iºî'FíÊ]´Ás�Aç…É�A‚âG'c1'Øè^ô…ÙÅd õ�ûáÍ [•«’;ai¥™]0	ƒëÕÞV·vèy#œ>„Iúè{…¥R�&¼ŠÈ¿NÊ�FÝÞ¬=ÊA'Îš0;‚\fDÔƒÒuÛY©£¶âpdá·‹ÑtQø
£„Ã(¤Q!s¨jXàÖ»Ê”‘šŸŠú¾¸'Š4h�Ñ}%K=rPètF-Ù²“Jahc¤ÝÅ¼‘MFuÖkOy·ú ®“ÇÏÄì1ÈÁí¨Ý¿°¯4Ë1óf @³ãÌ™4v6àuc¥D±„|¢|’ªS0 ¢ìHÜ'ÅŠV8ÜàMµ:­Ü,b|‰èXMŽf¿xæ6|uKR"uó;KÝìµ¤%2_Í¬lâÄ`mò>?ÐbûHêPÅ�àA–Ô}5ßQeåÚÎÓE0'™ê€ÁÂ©@dàU»£Tæ>°cÇ´ìSý‚l†)x¦ö©Éó%+cÁïý¸XrµÞmq:ôB…èIœ¶†|‹oAMÐ3ÒµÙd÷ãùïùn>ýªÀn&õxâh\k “D<ëÅÌegÀ{¶›™Î]a\9U<×ƒ?ÀPûsà)&&’à*Ò^u²ñD3H0àŽ�	¨J“pwÒ›YEùª+�Õ•Éêº7IRqœ9½ó±±«+B³ØàÆr×K¬EJÒÿÄìžVì+Òd}»æ‹.ò­,Hémò€ï�ß£�‹ÃÊCrÖr2ô›jFï_…Dþx¤©ö FuüIz‚_ß®7Àtc€OoÕ-5ƒ¿ÁWR¶XÖÑAP�{þ\8Dç—·ÌåFâå=Ä
jóuõ8GsÈóýÒû�Ú^ÈB¸íiÀÅÑf#&å
{"y¬Æ$çˆM“Jdò5 ÛÊ”v…©Ñ*%Bz/i¿ÏôKÿ¿`äHÕgGzIÚgñ:«áX³ççåòZÌË¿¸
&ŸüÖ_Q±gb‡8s�è`$ïÀŠ×!öâ�Ñ÷·pzà>Ûˆ¾¢dÀBÑà)›×mùú´Á™ÝñRýëüož1;»#Ï|Ý;=koD•¾ë}ÀgÚ²ÖÈ[»3ðZþ‘1‰Æ9YyÂ3þQ,qß=OU¶â’^Þã'æ:�ÚéÉU£Ð,�K[ÎUë:8+ÛiÚIýhv.ã�Òx³+ÎÇ2PDÔíž}‹Wº]G5±„EÓ›SK[YÏeKAÃUK|¦þQ{ îî|2¸¯ÃÐ}ÅBò\]UfrfFÁY9Žê–žr5•5-
ý
`râŸ¬mr.Ã14ÿij“Æ)Í²º­,h�/ÖãSõ÷h3:Pqp‰‹y°~¡GNq
�í]¤çvñìLD¶6…HB§Î÷Â��yooØž°R€U¤Ö—9ûDÙãÈfÃøóm‰‚ …¥è<èW6šêµ-®—`Qˆà/Ì§=FŸcÜ6ž‡ŸÕD9gMMs”á“†_ËSNT…0Þ<	½²;¹3úÞàÂÈ7aw)X‡‚k=Èz"tït¾O[Ðò)2–¦„kéÍGùQK†Ö%CŠÞ0(´DÚËë�h™	/ˆ&ÔÎ.Ab¯ Ä)nV›�ÅêƒŒ!–`p¼S½í?y|]$Ì*}Håþ_ÞÚU4ây*w¦ íÄ£ÿ+Z0š;ÞËfSŽ&I ÙÔˆÎ±¸[¦|ì¤T< 1�B‡™Ï‡ÏvÄÊ_@.†’R¾T½½ñLÏŒ[\œ2”¾Å"*–­ìß�áZÞr:Ù.å×³‚û¡L3cÁ]+1MrL7}L¼¡&È0'LÎ¶{ÔŽQ×’-Ñ�2gÎ\‘-ïšÏú,ÿ®¥¡*	4‰ûÞÓü¥Œæ·v)ã1yÎ3Z‹ç±.ÎC‹5£q(©q¤Œ_§0eAMw	x˜D–¶x+}{l‹ç3¯xö–5?ÍHÐJ¼$
·æúŸ�
 uaé®(òn«Ü×ŸãMwžŸ«:»S‰¿”c¤?ë®?œ>�C*‹a-¸RÝ•ÖZD4˜¾UÖ»ðŽñÿsÝîÕÑïåD¶Ñ|D?û¿kz‘¸2°ß·þúîÅÚ)Ð–{”úÊE¢KBï9FÞòÆl½wÃ'¶C¨ßÇshíû–r0[Ÿñz²÷nº86ÿ†€*|Ép¼5›à‘•Š²Ó‚i�bp¿Jå0õGJ!¸§'E‘m¨éMÒ­¢ØËòrð>Â‚¦P§ÚÝ8úÚÜXrÊãà¥ˆ	K(ªFÞšâwËœH²æ¾Ž(ZZ®v:'j–t€DÕ®ÿÁË	“Œ:†¶¾"ðÚfTV–Êh¯WWÂé¨ï°ú¨fœKš|¾¹<XÞAöý}O¡Ó³1‰x­'ZÚÏ6;ëÇ	0ï6&!9ÃS“‘L[áù­·ˆ7dDzg[yªîIC ³qÃÌ>™¯Ú™¼cdQ3Èë‰IŒ`+;›
Â¨¥±§€&«ª¸¢"sØ«¥LDç+œ´uÞn)¨Gž:�­–¸	TÂ9/»ƒOÍš(›!o†»ìZºlÜ� gµ&R=_˜0åˆ:š:òy€rÎÄ²‹ãb”· ³·ºÓTg(_<ÕïêÙ‡Úúô {ÌÝyƒÑž|‘#-.QVÊÿá4VV÷‚Oý|”€ÿMEi–Tþd@Ýþí/Lt€}mX“Ãˆ‹–ŠÊ©°ÀƒÒrw�öc‡kn°|àü�ÖŸÃÆOÂºéŒ
Ò³ÉYiˆ?Mž6ž�¼h¹‡½j¸:’˜•àRj)Jª«"`âxŸž_�`Éxujw²´Ü‹¿ #ä¼™5œ^Þ+Š2-¨¬Õ¾ÝúNHÇÒØÉ·BíÆ«:@,A‚ú‘Úºƒßh)0Ä”�¦þçìGr~I? Ód7 íÒ¥<wÎ²¯ÓÎ¡[8=| —Þ’<k¿ëPj*cl´_Hqç	u2x„È=Ô—{gYtŽ=Á[ƒLíIÑÕ«ÀRÌ*„–àù)sgAl¹æÈ-xƒçñ¿³ÑófákY"ÏŸ>XK¸¶¹ LäƒYÍ`Þ®à]<sL;Žjòeñ·®¬Ý€Øm¨*W"w<Æ|7&±ª<yãMôÛ{Jõ±I•ÒR=íÊˆàó‹Y\r5o@€ È™©ýVð“K7)Ìœø}/³èƒøR6¤Pcíƒa·ò»2D³™NçÎØô�†ó2=P¿wÖýeœÿ®ƒTø   CAÀ[ŸÛ@  4¨§ ôŒƒD±Ägû    YZ  C(      C®ZTR

SMP4      >½-b  xÁI¬®ézäûy›¯ÿþf­µ×Ú»ê´UÇÆ‰±�Ù>6‰�„”Æ„	  d�Ä ‰	£ã3$¯‚�@!Áˆ ()1HAã¸©Sµkï½ú¿ûš·yn®Kþûÿ¤ºÚ{ïªª­7Í¸»Úuûq³¿Û~ï;ŸßÜÜ}÷nýö»?Ü~yýñoþì»·?_ýü_ø…_ýÞ/à§ø3øó€?oñÓðh¸ÂOƒÃï‡ÏðÓÐá§Áãgÿ×ÿ†ÿïÿ¿ÿ÷þŸüýô³¯?ý“?¼ÿ~öøÕ7ò_}õ‡ï¿9~úpùtyz9ÞŸ�ÓóeY.)å¬ªbð[øÐþ7Ðß”B(ÂàA	¡F!P	¤¤!jT("0¡‚( ÐP3xP i„<¤A
	€	€@T ¢<(€�€�€P$Àà 4KT’P	BšpQˆ†
0400 € ‚Š€¥P…aè 
QDÃ[HÚ.PA@D‚3 j!@@  	Q	(QQ�„7 …€çŒæÕ¥caˆRr)ÒoKf+†Æ†ÿ$' 3•"?
5»ìlk4i†£ñÆ†/A²$§ÖUVÊª…ŽÖ:'á' �m2ÅVlŒ‰�ÊÕ0–¡§v^[×ídx>^¦Ø¢ž¯æð9(R4š
=qZWVâ,¥T«EÕ;å²²X‡š"E Ì„5[Ç¸&¡w° …)FÓ4{àr8µ6]^tŽ±xi,�Õp
�m›.çtŠ‰•wbLØ€�R–¢Õ06\¦ã$¶í[7ç½sFD@Ë%­Íö¦ª§—ç\ß8�Høh^²ñý†<Ï‹¢n*@$üó ¤è¢ê‡ÁNñ¼&Ž®xhèA�–Õ²©ªÄe¥x'^Mø)9}[U§õxJZUÎQ@f¥ôÍÕÕÿŽòþŒÖ©¥@t)«ßï›é|>bØ4­Ò °ÄÉîoF÷øñg¯ŸÆ~ÓÜáüôtŽoTÈpRâ3nü|y^.vl‹M"Þ‚"šWñu[Ïë<%Ôµƒ¥P$Å³ì?¿þø¸Ýq…*%|¢K‰n¸ÚOñù ]ß›D4üòšòp×åóãcÒý¦1T€¡E´¬jýP¯ózYL]YÑðRIf¸¹ª^_ÏÇÁíÄ ƒ‚X
®ú¶~<>V®œI"%8Y×ä6ß�xº2ŸmÝ3¨b5.S³ûþ°yýöé†íöjÄtøørñCç• Ã-HcrYánÌÏÏ/i×õ…"ÉZUû®ºL/ß;7Ê8/ËsâÐe# ÃRL±GSßÝôÍÇ_ÿ/e³«PŒ˜ð} ä¸˜«ÛÍðáÓÇÞ¶+¿Ò˜\–yxsUN¾N/ÍfL
€¡ÐHô}oV} õ¶2jDÃ;�FtZ×æzïNÇo�ËUßÙT„0zÉ¹»Þëéø°À�]2
RÀr^Kÿ®áóáÓœ†Í€b¡`>eÞ~ùù/üñû?þS`Øííæðéa9ôû¶Š Þ€ ˜Ï³Œ·wIŸ3º+Š˜Ð‚ N'õƒÏpüúýÿˆwû«”JBHÌÒlúåõ</ÒuÖ?‚Š¦Kî?ûN?Ý<»«±E¢‚á' �É\uØ_Éòðíé¸éw9`è@ e*¶Úˆ¼œçÒøÆ¢áH#:¯¥Þo1=¿¼Ä}»¥IBaž£öW¯§‡Ô·»R[  Ä|Áö]ŸŸŽ¦ï›B�ŠO3Þ¾Kë§ÏŸþñõîêjSÇ§ož±é2aÂÔPt]µ»¹áññýÉ]í·Š˜p”œ¢ßôk~<d³ïÁ¨AŽjl[%�3QÙEÂÏ�4¢ó²øíàáÓÇˆ7ƒÍ
0ü¤ˆ.Ëbï>såÓÇÇ³ÝírVQJhA e"¶äe:e4Mˆ†wPC)zAû®åÓë‡£^ïœaV1L‹â¦nŸ/OO:ô£+ …4œbª¯¯¦Óùévì
¦Óêß}Üõ0WÝlžŽŸæØ�=MR0¼i$O³éßíõüþS\nv-ÃeM»ý§ÓÇ's½©šd†[@ÎÄ~tq:ŸYw•±
†�)Ó4]ßÝ\ã›�çRíºœ¤„_AAÌSµý8|¼í‡Ñ˜b•Z�dˆM7Är>×TÎw (ËÒnÞ6x|}=›]g£-Å�)ª÷N/öMÓÔÉ€Pƒ”#»Ûíp>¾¼Ún[e
	A9œóî³ï»û¯ÿôS…ýõÕÛ
�‡‡×±Ý$)V)áiÎ¸ºnæãËéb÷£c~R¤,Óexóv|yüøzFwL¢”ð¤‘2­©½¹š§ÃãÌvl!*&ü€ÎyÙn®¬^^¾ë:‚J	1E\Ý¶x½=åaðv6¤A™¶c£çã´ ªša×•Óë‹C'ýè˜Ö)©©›ý›êòúx>W»Áj:¢yÉ6–åpÉ¶ª�)Â Í§|óýýòþë?þJî®wÈ*J	¤‘|™¤ýìÎÝ?Þ/o;S0Ü‚ É²Äæj‹éùéñ‚fsµÅüz|^·õ¨.¿
ÀÛÆÍi¹ôÒ[õ„Â 5¿ûÎñ³?úêýöÍ��ª %üM�ˆË¥ÝÞùáãÃÓ?z7Te¶ C’©k×_g¼^..ïêíÐÎËë:¡ó_€MTßñ4›¦m¼:Ð€«¢ÝCËe^ŠTf\š/Çè|ëÔZ¥£j3i]NjÚÎùbDÃ¿‚"KœüæsÁáÛÃåÒozY• ×í04óåå4»¾±B†ƒ È²&·¿äùáéåüÙ¦KÑ�ˆPƒ EÓIÚ»ëO_Ÿ?noGU5`ø5P *3Û¶Î1½\*Óxˆ€@Ê«vïZç¸»Y`¸A‘uÍeóîÎ}søöÍ®½éÜÓÓËéb®O5`ø! Ç¤í]µ9ï_K=¶ „o@ ežr÷æÚœ¾}>VW}døP¦Õ47»Œ—§‹oÚ¢”ðc¨u>a»÷9>~ü
¿?ÝºRÊK»k‡—³mÚ¶@Â¿	B´,«k®jÁñábû±Ú™N×óT¼oÄ
%ümP ˆ1Þnd=.Ý¸«‹+dhA�Q6»ÊërHè».KøM¢º.p›ÎÄ—Ç¨Íf¨Ýy9e¶}#p¢¡ÂtŠ²¹õ|><hº±MÓ×(çyEeê~hX.ÓèjHøA� æU»»�==<Äi³m}�á‡ @I«~@^s©}g Ñð%@/ëÜïÞÖùáã‡¹êûz]Œ(¸j´m7TÓáe1µëª1s}�¢ömE¡„ƒAÔE6ûíùòøº¦]ß‹@JJ—ª•y�.0¶ÙlœžÎY»jhGÍ�‡iéÛ^)€ùµ½ª,§ã™›ºïÓÎçËkÔªoý@Lk)}ÝŠ¡hèA�wtõÖ\^â…CÛÖU—ù�›¦µ`pÐ%.Õþ¶•ôúñY®û~-IDCŠ¨.ªÕ°1º×Ü˜V|ÑÐ�"ª3L?øz‰'™G´V-@Qdq}1§ç(âÇ¾5qž§9÷~Øíýty<Gô}•L–ð%(®¢õ0b™—CÝ«Á!#³Ù�yZ×iGWÛt>ÅT[Œ]kâ|:¥¶24–‚Í›±?ÌOe®¬Û6Ýz9¿\¢Ô®«4-çd»¶›% „¥ôÛnÌóù24£ó$ü+ ˆ–E�ÚY§iò¶©V4|	
�H[C\Ï§â]%UÑð(¢:§dû«ët:}RW�- áïƒDÀÖC[æxÒÕýXãëy©»�‡N‡U]×ŠJ¸E´,ÕÞI<Ísj}}‚hè@Õ™â·tç|ºÌC5RËWAk�ÑpŠhÒÕ¶ÛžyyŽ«¯gÔŠ†/@ÍÓ’ÝöÆÇç×Óª7U©9OÆj±á-(Â¬©Ø~3øyzZP7�‡@L)k.õvlât>]X×}—çù4Wuã « "Xù­qs¼$ñu5‚ºÎS1ƒõ Ó;/ëñQEÆ¾­JÆ´^–Êošm1ë|ŠE›f»A9.ÀP×ÑP)9E­oúÎ_Ï¶©Û** +‹mFŸ�óq±u_Ãipˆ²¸aôF§—i±®1®À*Q0ŒuŠ—§Í®©a”FK¾dì:�8ÏóDTCÛ5˜§s6vlÄ¨1Êr‰¹Ù÷›|<=dHÝ÷e½œ"Ø;/w ˆæyuõf#Õez¢öCåE% HŽuoë¸NSv�iaT4Ü�"š—ÂzÓÙ²ž/	ÞÚzc\Êhãk8•°‘�X]Ùqz9¿²4¶�P¤,³ñûë&¿œïóÔõû]oëérJÉõ�‡Í€ê”¥ó�¹qµ3	=€kY}·ß(‡9÷]7ÔHLçy6ÞtUíòñ8«Ö¶� IV©÷Õ¶9_^›vÓ¸2-'xgk "ã\_/²¦E’Ç¦†¦u¹×6ÆÒ ÐyÙï«fy}yQÓŽ6Š%™c‚í›&¥xV:7ô��œã�Þ5&Kè@-kLÖï¯Nîéá©¸¶o!2üDNy©›7u™N§'a³©`:àZ¦\m®?ÃåþÓúÄºê×(†á-˜WÛô#Òúú94;W=g/Ý¶V7—%ÒT›,áç@$7©sÛ*¯§ã1��ïhi"S–Êµ°YÓ²,Îø«læy:Â£ª­Ð˜pŠ”¼¨÷}ãRœ U][«áÈxÛ·Ðõr6@=4kžcNÆVµ“òYnüÎUk>�ÉÆ·ðêuZ'Û¼»ðôðôŠqìÅF”hºã)Ïâš¾·rÌ§Å–Ê9GcÂj´ð®wv�1ãm-Ö†¿Š”²ˆ˜®²9�×ÕS9~D²kA»ïöéxx=zoZgA#î 9-•jó:;µ¦kÃ€RVµhz¬ù¼®¥2VX¢…­ÚÊ]ÖÓRt•3”ðs “eeën¶q™ááZÓ›×ª¡µª1Ÿ|¶2ú:r‰q­¥vµ8HÀ’L²¶eu]‹_¡)ás�™@å7µ[ÏÓ�¦©¼3´¢ˆbPµb¸ä9yk­¡„w ²,DSµ�éxš�ëª¦xJø!(’Ë
�®òÀ²¬Î9#F4¼Yf¢i›aÑ).ScãÕŠTxÓÐä5&ÅÐUUÒ¸&ë¤#a„jô±s(iJÑ W©„¿„RŒÔ­¡‰k™Zo\McÂ— EUç’9l®{.��ÑW°HøE�ZJ¦T®ÖjA.Åßˆ+¼äB'Ö~$Hd™ÔÊU3_Nm#-,Å	× šcq¹E­%žÖb¤­­!ËšˆM·cwYŸÒdW‰Uˆ$üyP‘]†¡¯ë*™Ë²ÞŽ¨Š+,Êºj|ËšK‘Ê:	o¡šŒ¢ª�a¹¢¶Ö9²ä¬Î´©J)­ëéUÂ;�Èv»~ãT/Ë‘:ëé¬„[�ÊÅbìº&-OK¶MS{Ø"&üu(�m‚‘®vkZbL•Aeá,$\�J%Eç|Æ9®©Î�STËºóV.ñ¸š�ïZU¤<¯­óM½ Ok�•
ND
"
|ß�8ŸÎÖw]åaÔ0Gªí\ÕçËñ8kU7•Q+%Ï%¹fßùÓËáIªºj�X8	-¨9¯Ž®®�ÉSž¬iZcP¦m†3ÅPK„Týœ—éëm%V ¢á –d_#¥Ädµª]ÅÂÑX(“Í¬»±Ó¥ä¾ß:\ÊSv�
Ö@$\�„æL:ç­¤……–ÎJø	HF¯ô¾2RÖ¸Ôè›Ú˜´&U§Vœ¾‹à(¥D+èúäó'ÀJ-ž0®@‚ª¥d4Þ[åœ£1Æ—º”Ä¶¿öi=<Ÿ*Ý˜Ö¸BkÄX(U‹¨ÌF{? ÎËq-¦k�5*~PIÉ9y×4Æ¬ÓTŠ�7"¢"�ðsP5¯®x_æu.ÖÖ[§fŽ)¢T¦2°¤¥˜°©PRSužšf&:+µ¡€`ø¨©¹dƒÎ¨*Y‰qN  *
¬Š:µÖYWžÖ:' HQ!„@†¢n›Žy9] ®­,UÅ@€Ð\hêJŒ&£5ÎYX"_lS™Â%®4jjXBBHMÎúÊ"—¥ˆÀ! 	¨P(@Éb*'Žy¡Ë´°�ðÏ�¤€%g¢vN4¯ªÖ´Ö’ŒuÆg�ð#¤ÝÔ€( 
DH¨P Q¥¡¤@E!@C!*‚"ªB@¢ Hp 
 A€€ )¤(@	H(¢ÀÐ‚"
!!A�P "P@JÀXkŒPsÉE„E‚" *€(DET P
ˆ¢"*á@B¢%T`DŒª²@¬:°äœœ*b­óÖˆ–\
ÅxØ¤%'S§¼´ä\2 ! *€„
ÆKj¬Kœ5ðZ}!Œ#Á�Yb­š†9!ÓÒ/c–ð(T-™ÎxW4–gÆl$¸bÑT%’¨èÕ†· XrNµí�•X4Jëi$t X’M®–ž(SŽâÅ;ë$ô ™Kìlë³ˆg¡SÕê°BÄÁÆ€ªIª¦Rå%—\WÓHø+ j^X»Meå¬§*màªÕ$“DgúÚ&,1Y©«jÒšRÑª®�Uçœ`¥‚sî@�j’"U«¦ä´ÒÐxë…áï‚fV¹jÄ§5FãL%Æ„ÿ ËŒzc›�g›Ú"‚©d¶×muZ—Õ6uÛvbÖõœPÉØ´ñ0-¥K0¼&Âº¡2iž&oëÚ‰Ã
(sF³¯Ýq:œµ®œÍF4ü
@×¤ÍUkÊÓq®º¾10¼”)²ÝoÛËôp‰Ã¶Qqª¾)K*v»ÛÊåþu5£w„PEë²Ø»[=MOÏGtu×wž—ÃƒøÑÙÍ¾ÊççeìŒ…*%Ü�4’çEÚ«
ÓñéÕvcC
þ@ÖÈa;´‡×Ëóè6^`Àð÷A \WµÃ›9=?ÞçÝug#A¨X]Ö‹½úîîøüþÛórU·ÍM�óãýë¼A½Ý�z|ý”p3v”0Ü� àRæöÍÞèéå´¾íz�ÿ1H#‘«ÛÜ\ÕËËKl¯;oÀð  ¦¥Ôýµ-Ï´�•}·áËóóÑô�›(R`Â¯� ˜ÅnW__OqS�ÂBÅ–é’voÞÂœ~vzŸ†v¸¾nÎç—çÉ�Uó¦Å|~ütjº�M î †’ËÂáÎ—××‡³íºš’Èðw@ :§\_ïÏÏG7= R`Â¡†¢qÑýÛïúû�ß¾Ú«:e%ZbœÍõu}˜ŸïÏ¸Ú§«”ðë (šÔŸíýñøn[µ €*†q�îúm�õý·ßü›¦ÝUMÉO�9¿‘jÜÙtz>¤a?ØŒD†[‰\°½¾NÇ×c±û¶a~À|¤m7n¹¼Lžý€• Ã¯ƒ `]³oªãéþù\õÞ�]{Z__/èzC5`ø	Ô@ó)Úíõí|<ß§jß#S
…Rô\vß½Þ^~ö‘ØùþêZðòòÛ¾ëÞ\-ùðø$~'B•Þ@TÏ9oö{}9? MÝ­*%ü ëjÜµµ¼¼œb»±•¥„Ÿ€4’çÙ×·WÓùåþÍÐ
U
Lø› D±jv›ëò”´í*GÃ/ƒÁaMÙÛÝòúò’ýØxQ€á7Aˆb-êú�Óó4§¶Š@HYKª¶Õ~º<]Ì€º½òãýÓ½­‡mIþ:ˆ®%ùa[M——çÄ®±¢`ø sF}íu™Ž‹i\»š¢ §©joïòåÓËSé6‹~„¨.	Õn×žç×ÔÕ›}_.ÏO¸±S)":�’ÎIöovééütì7u«	_€ Èœ§qÜUËññaÝ7ÛÉe�¦E]·wñðòŠ¶“n¿o§—ãýk;lœÉ Ã�A º$6û-Îóé5�²É®�á  ¦xî‡·[¼~útÈÃ®ÙoÚãéõ4u—Õ‚’Ë*ÝÍ‡Ó'U·©Z@™››—ËË)mG t^á¯FLž_Ó¶ë`ø §u®w7öòéãã´Ýo¼
Èð‹  Î1ûíVÏ¯ièz~$kŽívGŽ“­}=nßÌ——§gÝöC1J	oA°*šn›Öøz)Æºúm/Ÿž_ÑºÍ86/óëSÑvl€""—„aã2=OFã³Ñ¼¨©zÑtYŠV¦y3j<Ýû"»ÑfÉ …k‚Œ­]^ž'ÖðõõVž÷Çñ~Û¼�Ìáø|`ÓîwMy>~XóíØaq Ã  Ni­÷#âÓåbÝX×É€È1k{{ÕŸž¢÷C+ÅCC
€£mßE^uC�hø�’«ß|W^?Ý?U·›.&24 Dób·×7ëñåÆºqs}¯�Éô›ÚPÁÐ�€d,¬7Ûý|z>×¤)ù”\s}åO—§ÛÖÙbDÃï€Åœ¦nw[]÷çS·mÚB…H%J÷ö——û'¸±+�0€Í—ÄþÊçeyšé‡:£Hø-P \˜ê~ ×Ãi…«ýÍ¦:_^{Ý×(F4üeP Yu‘öfäøt6ÃÐb…o@�p¥Ú~@^ÎçèM+>‹hø%P XK´õõn=^f˜­‡Ë¾”)•v»Árx>4èz
 áß!Z–ˆvlÓt:/±êª
EÂ 
�4aØÍty}eÛ6†F4ü.(¢:çèÇ�[_ÏÓ¹ï|]hDÃoƒ$$[�­.Óá‚¡©�høeP H)c»íãz~]¥î*¯F4t DË´¨Ýmb><žÏh÷Œ!SÌ²Ù¹QO¯‹˜¡v€ÕðP HiÍãv;^Ž÷O¸ª;Z5F4ŸUû]�y:LÕ5ÆÀjømPD¹¢4Ãh¦tžŠÙ¸°áo�áZ–®{çÆÓËó«Èn¤Uc$Ï%ÕÃ¶vÇãñ¬C×©¡hèA-kf½kÜšŽq‚­œÀ†;PDóÌa×bº?ó¶êPhDÃï�)—qÜ¾9¿œîóŠºÙwHÓñ2[©;c‹„[PDó%—v¬6Ó|˜–Îyoi@‘T–änî*<ž?®i³Û: „Ñ²¤ìÇq—ž¦—s²míA	¿
ù² ºÚûåét„o‡VÃ�A‘„EÍxÕöåð„­¯«lSøs .€ïFäK<D¶½·êL Â/ƒ9FúvtX—#K-Î‹¡5%Võè‘ç	±³•…˜ðïƒ"9j¶mßÊù2ell->Kø·@‘h›¡wy^Ï‹m›F$üG`I©8·éJNÇLÓtÎYØð_€L(¥i¯�§Ë)Û[Øð¯‚\¡Æµ™â��ªòJ4R7#‰K:¡2Öœ	Ô¼$Øfúx>>£ômg­Z	ï@�Ûd�g°®lK¯FP£Qc6M7¥uº˜Ê9ëDŒ	Ì±Ð¹Ìé´œ‰ºqÆd‘¼0£º~Mçs,ô®¡(2­1}SÏ%­K�+ÛÐ:æ<£²ƒcJó	pZ³ÉÆ„Ï 9&z?:à|yÍ‚ÆW¢V áoƒšhÌPµm‰Kš@k¼1FÂ/�šg…oû¾:Åã!¢cmœ„?æXXù¹¤ã2W�1˜h¤­FÇ)=çRYc=hLx²¬Æ4¾u	:•I`|ÓKÌéUcXÓS@d‰p�ïM.ç5©�JøP�—¡®$•9ÏÆZT¦Š	·Ð’$ºª©Dóºfaí�'Du5³¯úŒç):_Õ^+…"«‰¾é³]Óa*"U_;ärY¦
µwÆ@Ë'ÍÐä”g�o´Q‹®ŽUSiY2�zÔjLøP5/"Æ�ôy^c¬¬“Z `²Åbð]Žñ¼N¹k†ZXÖ2Aµ±jÃ-%c+Ûøî$1®Yj‡:P¡±ÎÞ»Æ�KÉ�5ŽbB’ZJ­ªªAŠ‰Ñ[8«V á·¡`I²zçûd4M3+[w4Ìó’¬q¦ë$ü,%ú"Mo\Á‰•úh‰¦¶mçfMË¢ÅYoáŒ„
-Éë}•s9ç1â¬•0@Q"`eã½[c¼TÅYoa�„_‰Â²Z¡¼Ãñ´äuea %Áš®v&¯iMÞ84*Í‘5šÎYâš`¥2bþH-9:Ó;gS>'²²¶.FÂ¿ B‰l¢cÕy§ù3¤AM FÄ„¿H¤*[{]SZo½ vP!Œ"ºµ©ªŽ’¦U‹¥3pB‚¡`Ž­zï°ä)©�­i)~•ÐE}Õx–uÍÅ‡F�aÑh�ôu¥E×5ÃXx¯" €ZP( µ¤bÔÞØãtQ²qVŒ1€2ôP2+Ö�¬©dqR‹Š¡˜°…
!Ôè’V­›Ý\f#lQ‰!(FÂ¿Ò€Be¶³ ­vù4ŸV_IÃ*ÚB#d B šs1�w&'M­óÖD *¢š£¨í½·yI%‰:º"FÅ	‚Ê’¢HÛÖ3Ö"ÆXÔ$„áÏ€PjI¦8_Ó¥¼0¹b*8ˆ >‡BAJ†-¾ñU^¸Z ñ¨Š	T@U+ikKÄ3�Ôâµˆ	o¡† P‘Üj}Õµtó|)ëŒë ¿¡2çh½išZ°êç\çâš4y•J`(b@¡Š�¢¾…3žVeTÆX#Š’V?#:¯Ñˆuô*Æ•ÂPƒPj^�­ê¾B‰Ó¼Vh½sc	Õ˜ljl‹Â¬koœ‡¥€%J2®3¢SDjlí™çTR]�j0Å5ÒÁ[µK [P©%9Syç¨K)fíi¬1„„?�—ÿ¬ýULÿÌ¿-ÿ´ÏYLM
*HaQŠ¨Ø" ! EŠ 
J!‚"j”"E ¡*PDA08P„¡…) 
Q@48 * @�
A@@1*5 ªT*C@„0A!C›EŽ D¨F!C€JPAP &4@Š
h F B*hhŒQ0«¤Ð€Bˆ€¨@‚E<DƒET„ÁƒP!0FDÌ,
Š5VŒ�Ù+(††á/�™ÉUc�jD2FšäSø[`t<ûÝò¶}®^åÉ‹Çõ7ß;‡ŒÃËpÜã¶ìž�¥Æ&Þ~}=ƒY“Ðt²“ò^§§Ï�CÇÒÄð÷@aÉ99ï+�«æJjõ  ÇDß5µIÇ)¯#:GcÂ;P�stÖõå’2¼sj�Òˆæ¼XéÚ*çy^¬³V¬w  šr’a»A<¿>×®i�ÍnA4%•aldšçÜÖ�Ó:KAË"Æ·VR™W'm#U@)~-u×#-Ë%»ÚzVEÂBJ^•m7èåøº²©j8€ ®±HsÕ¯ñð´šfã½:Ã-ˆ®1awµ+OÏ/g¿okÉ¶Ô*•Iê~ƒÏ{ã¬Éî@¡HNó´ó¶ž¿ùVª›-Q(¢áPr:²ýÎÍþòðÍë«ùl@Q€¡Éº–zwÍåÃ§WŒûÑþ'¢\ÒZï®w——ÇcÚ7—
Òp]æaÿÝñ5>ÜOÍíÖ‘· (’Övß{ƒ·ß|ý'�y{ms
¢�í›6Æó16]íÕ‰†[P(Ðó¤ö×7öý·ŸþW{hÀðË @ŠÐ¸i]Îäc½ûÑòÇp�»wÛe*[Ò9ËÍf°çÃœ¯º®Zdø= ã*æÍM{9|:¶ÍX_ŠÀe‰n»ož_ï_ófè`¸…Š‘r>¯Wßùùk|óÕWÀÛ;'(…‚<ÁÕ#çËÁúº1bEÃ-(„ÍqŠ×Ÿ½)øøá›³Ûm¢=`¸A Ž¼ùÑ¾ùöŸÕ]%„„‚ ¸ÄÙ·vyzüöln{'YÁð{ �äiIÃÝÕòúü1¥Û±Ÿ„J˜ÖlöÛÎžÖô}	0ÜBÅH9/ã÷þl“þà�þd:¼Ý]@Â€+Pïi—õ"R;ç³·PÁtÈöóÈË}u?áîÚ-P1á¯�B!�2ïo€Ëýûg4›."+þH#ùQ¿Ý–óÃã“^íA	ƒy-ÍöNîî×êz  `x‚@ZcÚþàÚÝÿÁ·²ï®“*d-®Ü”Ïû¦1"`x
(Hk®®¿³ùðõýÏÎ××jK)6V&ü5€Nóº½ÙV§�§acÀð_‚4’O9õwµäã‡œûm-T¨ˆhœÐ}¶ÛÞ8sØ¶6`¸äR°ÛTéx9¨m¥P(*³Tc£<¯S�¶Ò*K¸¡FR|‰»}üá¾Ç÷ßr™H[*®@
8²{÷Y9~õÍ×—þoe]@:P€²À7MUÒy[5à²¬ýv×]îŸžž
*ý¶;Í÷?û0ï·Œ4J	- \ÖÜ]ï¤<<ŸÖ=¤½+ÿáã{ðæ¥ *¶äi5Ãi¦—×]×d!Tl¹ÒðÝï#ó³¯Î�W·¾ÌVÁp
:Ïy¸»Þ.Ó‡o¿¾éºª©m³Îç‹ø¶—L¬DÒzJwß‹÷ïÿéuGÓ¹º[.O“V•ÔcãóùgßUU¶!%.êÇ�]ÏÏ'qÍÆ.dø7@�dsßKYÏóYœ¯è(¡”KbeËñðzÁ0T°EAA‰ÅÛQäxžV¸ÊøL	oA Yçªßïõx¾·u3Ö¸¸¤¾ !%Í¥6†ËáŒ¶êi‹BtZÒæj¿¾|órlÆ ÃA œW±»=ÝýË·m'¢ Ã- —ÂqG{¹\ÎÜµÎ&w €\Š©G‘çÓEÅ×U¶Ðp5M‡Òÿðç’ùƒ¯¾žøvãÅX,zZi¬Ô %f/×µœ¦¥öÞf#þ!H#e>™îê�¯_Þ2ixcT­RÂ¿‡5/U·÷z>\ì0vµÛ4˜×gÅØµFŒhø ÓÛ›^íÓË!6�t××M5›MSm{7çÃËŒªõ0…w €Äµ®®ëúüòºÔc[­¦((H‘Ü\<^/Uklµƒ_¦Ó±Tõ�iECÐeeu5hõt<‹ÙÔj’ˆ†‚–5Ïnlmyx¹À´Öú7Õærxy^6½­7]�Oqi›Î¢¡ƒC\VÙ|¶Ùžï?<£ÞöI 0ÏIú+›–×ãÁïl›Õ‰†_ƒÃšgÝíû¤O¯—XÕÞ½Ãt>­Ú6žB	¿B4Ï«éwæóËiîïÑðH™—Å]ï*]Ÿ]Ý×-(R–evW7»õåÛ¯2¶mSõ’æó�uÓx›$|	B4ÏY‡�çt8GŒ®8ÑÐƒ Îµ{{[]^>|,þ®ó&EÖxâÕ÷n?ûôáO?E\íÚ`øgA¡Ié<§êÍíUüøíñÐ�Û¬RTÂ¯‚Å’¦j¼Ût‡×�Öã© ¹Æè÷7M\žOÏý°CEÃïB¨—ý›mã.Ÿ¾}à°s)døu08Ä5–áówÛ‡o>>>ºM_.Ùˆ†)å"f7Z³N—çMUD4ü Éå¬æî³îôôþgÓpÓíl×,ÇS6m]©WÑð‚"šgJßuÌÓ%yÛy?EržØ4,ér­*XIÈh7æx¾”Ú:o(á?!Z¦Ë4ÞÞlýãû¯Çn?–³jø—À-Çº•’ÎsA][±a!ªóZìÕÕÛ)�?%Œû-]1F¨ib¼~û=>>,çz³ï7u_.Ç§u±uSQŒ†[�\–Ë°»ÙŸ_ß¿ ï7«…„ÿ)ù¼æîÍàŸ_Ÿ;KR4ücPódQ÷Qâ²,F:k(á÷A&·ÖM�¢9­‚¶gÂ¿2!£p>¤d\ç-„á((‰¶©Z§œR„µÆRÂÙœêRoþÿ‚àœ×Òm;ð;Æl¾v­ÝÕ®ªãkÉæÛÑ#®-‹Èã ÉM‚d2$K$¤ˆ	‚™òCù lŸ{NUífu_3›ñò<¾í×m	2tÖ·ô» @ÐvZ?ÍŽÛ~‡±0Iÿlu—.öƒrk«˜¢£WIß�,(ðÇÛûõü6¹¡Š8YüâušÂZ¶ÕÄO,Húï eQS—NšÕ;¯pé·ÁVÊ0Îµ½Þ®V¤ï=àÒ?YBmQ‡Š]+œ‹”ôç Ú.Õºãa®—ó¾Š;8‚VW­n
û²":é�¤29Ž³mK>-1…“ôK�@«;ôk[Ð¢óÞÁ+`­0¢˜ËZš—~ŽµÔŒÚ¹(Îk:¢YÖ&Ý¤®Õ²	£FQ‘ô»0A­E$ŒÎX.uïÐS5ý{„±íÑ|Ý.[i5ˆó ª¬”~šmm—õ‚Á{LU!éß‚`­«¨Éû¶ì»‹^=EÓo‚ µ¶kÕaöXK¾9ñ2Zg�ôhV|��ïDkn £‡WÕô	4+&<úƒ³\ÖÌ{¯
I¿a”Æ¬{ðýà¤Ô¼4ïâ8¶Úò¶íž.úmm*>¨*aÉ×¢Sn­˜3'Î©¤ßAZ³ŠÐ…f#¼6GƒÙ®9ôa‚ÔÛfââÅÕšk†8u‘ÒŠ+½Ì-Xkµ˜§§*éšÕ²Hè&-íz=/!z'JX­”BÓ¶<Õù&š˜ V3=†©•ZN;!½t"¤¦?P¤ÙÊŠ~z {¿½_6�¡ƒx@”ô`Â¶;ø®WÝZÞ©�ù
°µš½�±³V6d_¢9Š¦¢A›µ-0t¡kÌÛN‹~îuÍ‹7ïU¼@%ýhÕš¸Þ)™Ñ N¨f¬›7ßõ
.m…Z@pÐô/±…º=\¿;hý79åa{@”×_Ü$}ÑZ-McŒ`µ"¼yƒ‚Íª×A#µ¶Æ`ê¼‡ ­4"vðV­ÁõÞd+…ÞuÞ´4P‚sN�ô@Jk96éSk–‰Î‹ŠB Iw A´Œê0…ÞÚ-Ÿ09…B¨M3H�`Û¡C§ý’O=G,VTWBô
¬Î©¨ ’þ­fßyGnµÑ!4×�¤ RŒ,Ž:"¢f5mô
¨’þL� ÄZ‘Æ:C[n»a<Ô>·e«A†~rRí
S‚0ƒªzˆ¦?A¬­1¸ ‚�G§Ž51!HC{�®w¸Õ½4Ç †ª¤kâ˜~¤Ð’‡T­Uüì½á¼³ÝXóu-U}Tqb„
 é�AB[¥xç}³Rµ4  ‚†Öªyé<4[-Ê¾ÞÊ²­*Ã8*uC%�£óBÑô— ±V<ÑKgno»yÇÖÚæÔEqÂ–;P¼ˆ¤g�€›Q|¯JîÂ`*N’"”ô=H1Q˜Y‰Á÷ÐZÌ|• ³@5ýƒ@kÍÔŒ¥f83	’<„˜ ´–#cèv{ûoø×¨üYú AÐ"LˆB @ÄDìôýþ÷ß|M” –€"&1 � ÄD@QTÂ�$BQŠP1�¨‰iDƒ@�FóF°¶Z­‘Š  ª‰P,yP„)€"„Xò @
A˜ @bI(€˜1‚ @„ (� 4ÍhP­dÀEU !H€ @BLÄD˜"Ä’‡˜ÅD`zV�ªÑœ‹Ž[ÍÚ™KkmfÁwÚ�kC4oÎIA±`ÕÄ{çØ6iÞàÄ¥g¬¥äà»^tßnºõêßJÛÊÒbßù�¹žYÕ»€ L#Xe×›w÷Èã×Û9^�"÷ßZ«­JŒA˜m�5¸CD«u­ïÞ1ÝƒBkÙ5™½×²ÕÜ<œM™~$Zi¦�ï«ì¥V×†è÷\®œÓ®š•L>¨7¦¶Z²s±÷fûnMè%P˜>�BV)¹÷£kÌ%Þq
cÖ†!Dæ½ìT:(LŸA¡eW5öŠ½e‘Ø!6I3(@uœfÔ¼œ<bç$4K
h›3=x¿Õ­Ñ9	PP³YwBÍç›ÄØ‰‡Húç ¤Õ,Nï|á5_ è¦‡‘xy»,½Ný}¶ým©ƒI#i–åøØ—·óiµaŽŽNÁôP±ù8û}¹¾St¡©€h9C‡AC=ŸW›|'J0}ä×ÍÎß¶-ç1/
¦	ÀŠUŽ‡Vo—ÃU	¦¿ !Æ\[8Î°ë¹ÕyÁ „¢–UÆçÃ­\¿¢M}ªQÒ‚©yãôk÷÷¯?ýøZb'úüüàþëoÿóvŒÞ==·}}ùºãþ�RÅ(i AÁ².wÂö«_}iú<»f Óg�*õ¶æùù(?¿|Ùy8Î¦lÐ.Ã§©ðýKÆxì…UMA@Š’ãqÆºœ–:E§2M  ›…û»Ãòí§³›§	j ÓŸƒ Ú–1ÝMííö¶u‡Øk)Òò^äcÿ´ž^¯<<ÄU€éA mÏ6Ü8Ý^/]ëåîÃ=Ö¿üü¿|'÷¿~8½}Ù8M£Û�`@ŠØ¾-ãã¯wíõÇ¿ýÂ_Lã�0Jú‚‚mÕðñˆåõ§·µŸÙR`ëÒÂóNï?ç}8Nû€é ­�óÑi¹žª»ï†ÍƒLH•Ò6™ï»~{YÏÃ¡ÎLR¥ØýÓ—óyñÝT]…‰Jm[ë>ŒûéümñÇy©Bhú€åŠþ±—õ´,%Äï�·ÿ÷µãŸŽávzûvžž�±%õ ØWÊóç¡}ùòÓ:Ì�a- %}A ï%>~FÍ__^ë‡C·f�[Ö2}~*ùW/—|<ô¾@MÏ  -—Úï»í¶¼õ:ˆ€i©R×ÝçÁµëíæGTªQÒŸ‚ ,ß?ŒÃõò^ö>5n{îî±¾üêÇyF(¦hKÞçÇ—·—oÒ7©Bhú;0*õ¯—·b&aþìðòõËOƒŽáÐ�ß~ªèïz­"šz�"5o:ÿâ;yûùeÁóáPÍIMaJ©mÁñña;¿þ|²é)V
)à¶—ãã!¾]~uÊ‡N‹˜>‚cfëï¢¿ÝÖÜäà �LLamÙüýý¡�–W�aèª2ý ˜é0víú¶¯ý@0ÓÇûþ´½�ö»éà›)(,köÃÃ´žN§Ë±›ƒ‰%ý hk•0rÞß—=PžŽ¢?¿~ùÒßß÷ÇÇžõôÅÚ¡Ä¦e_åáùCýúíòÎù[£¤� U+7êÓ¸_¾|{Ýã|X”–Íôá¡Û//Ë{¼¿S!Àô„3kw×»¼.£÷±L  Èc?íùôª:}ñ ÓŸ‚ ,7™æ¹Þ®+1Í(°mG|>:¼½½Bç`ƒ¨lÞ?Äý}=1NƒÓï� *Mü½è²\*ºÞ‰�éƒ ÚBïâR–BÅ…pfÜ8�yÛoÍõ] K¿ŠäpªÏ‡ï§åüøòy|¸/’þÀz¦ë?„òþž¯wã¬ A°l­¾³þúvÅñq@&Àôa
ÖvóÝ¯M|ý’[×ùì¤AÓBZ]é§¾¿í—sÖÆùéqÍ/çw��Ýt°ò~]¶¾ŸÙ˜&ÒÊÚâ<„¼–«—~²ØÈô ÄÚÞB<˜nËº¹¡óÚ$ý ‚ZeÅáÃwùöã9Çç>f L?À,X§ð4ØzzÍÓ¡ïZ˜þPdí§çïç—½ÕC¼¸¯_ß~ü©ÿtwØ70ýæÝÇûy;¿~½­�‡»Ãéü7¯/×Î»áðÎoóróCè>|®¯×¯ß®xºó¸F
š}×n×K£ï«#p-µ»åöå³Œ÷�wxÿöã[ût7ÛK	w÷�¸|»x|èãæA¦¤m;ýqF}ûòb�ºîî)œoßÎ'<ÀôÀzYÜñã/ôÛ—ßûÃcD© ‘þ(–›Å)Æs¾\}ï	I#ˆí¥ðá)–ëÛ·&Ç¡“ª QjkÇ§óÛ¥ºÎš‚é{P Ëvxûåüí¬qšî�ÀåÛÏ§å»ûqMÌÈrÿÐó¼¼�!F×T,}Ù±ãçAx~}…›ÒÀô=1Ûy|˜‡Ëõe—ù+”\9x–÷¯ÇÐƒ ¥)0t¿n¸¨“Å*é1lms�Oëúrƒ»;ÓF¦ïA@ªmûáþ‘çË××Sx>ÓF¦	¤³²lm|œ¯o_sˆ~úp./½½ta>ÌÏ[;½¼Ú<Ráh©!V·ý³¿?¿ž¾™Â(é1î¹ê‡û·í}%;Ì÷£ìùío_¯‡cxzòËéË"ãa0BŒŠÒL»c°¼^Zè» õ¶axº×·×××M£ i @®Ë8~þ`§ó—êôØCLŸAˆÙjjïÛrÊ°î0uóz9�K?õžMÒGÛJv�Oýz}ûZÑÇéF'–¾Ù¹HütØÏoo_Wß÷ÓÓ',§¯_v÷xçM›`Û(ã4¯u]tÒ‡¶\NWï1ÌóØµ×÷[7L] Ø^r7?ôÛít]¡1ÜÝ=ž®ço†cæ é�€X¶ûïÆüúþ%Ÿ\8üÝr]¾]øÐÍð¦ *rëîæ€ÛkÎ!Ž‘BI#(b¶†ÃÛùí½È<DÂÑÒŠÇÙåv]ïüÝA·Ëé­âà�9±ô@�r�óýq;�n»]­’þ(�ŠÊánÂö~[‡nê< I?€«·µLg½ýôó—÷ùñà¯®‘é—  E¶Ò?ÌáöíõËZ�£~º›âåç·Ÿ¬ÏûõýôÕÚ0"€¤_‚kkáp¼Ÿ÷Ëå„ØO€¤ß!l¹îîø©oåôz«ÇÐ?|¹üøòí¶ý|ø¨—Óù§EîþùSl¯¯¯o[˜ŽO�o_õRÂxøð]êé§×œ»éyžä§óÏÛ5Þ?sUÒ3(luÕ»�ðvùºøã8¹Ž–žAˆÙ²Ww˜u_^nÍqžîž¯ï×¯/å)ÞKI•Öºû)”Ûûb~DÄÒ
�ir< nÛûZ"¦ûþq8]^rè¦QR-Óñ0 më¢}ßsbiE¬mT?�¹îk­�Ý‡!ÖËíg¬Ã]?Õ¶å}­}4O1€moêÆy«·íªÃä¢9±ôKP …[âzz;]eŒƒÏ^ÅÒ ŠX©Ù†‡ùþv~=©Ìƒ÷Í‰¥� ˆe)œç;îû×ëâº>
%yXi-Æ‡À²Ÿ)QG�ÀÌÒÆ§£ÈíçÅë»¢5y´r¡ÌOžËõ=_mˆwñÉÂù¼à8uèÄÒ Š˜í­ºáNC;ŸOq�N°íÝ}?ìÛõÚJŒ^œh9CŽwÚ�×÷’û)zÀYú+Ðöj:ôƒ­×k–0v
(PQrì?Ì\ßÖ]ÇîÐ °®…Ýýõåú¶`žƒ8°Œ¬ã}‡5Ë×õ.4€Y²öÇã´—÷%çCjSM3(RwXº­åèA]Kß�áÎæÆpty9çMFw}�×ÛÖZ££Ö–Šy<zË·ëŽaŽNLÒß…µ]DŽ!–zÝ÷N;Júh;†¹­Ëª}×;o’þ´ÜŠŽOwÓõòeÑ»0¢Ò‰¥@‘škÓîà‘—Û�ï�k’~dfSÛ¾¿—|'ÑÀbÃaØÏù„®ÕSÒ´�ÕÓ¡´Ûm?‹óã0p\Îo×ØÇŠ(`YMÝ�ñÒnK57'&éŠ´¬ÎwSqKÞÖ€1:Qj¦nêšÏ—-F]Sgé(bu§ôÝ¬r[¯ÅµàŽq�S»-Úû(.}™AÅÝpÛÏ±��yh¥Òûi’%_/Ífí§¡/m½65˜×ô{0ÛÌé4CnËÚ¢v�§éwÀV¨}7e·Õ\ªvÁ)%ý!Ø2Dã«õ­BC"*–fXA“¾Ÿº¶îÛ�Ö‡ ¸t Y)­çI–üjƒ³ é;PÄê.ŠyòVÖuwN‚:“4ƒl›!ÆI\Ù.§ÜdîçÙ.Ëû©“¹?¨å¼m§Œ.î4.·÷ZÔc œXú¢ÊU|è�jëíR<úÎ{Š0ý	¬ÕbÞuÎ´ÀÌ©8/’Ž`«™£ŸaÆ[.NÃ0tEòm_=û(‘B«ÜØ�XÛ~Û ½÷ª-Ñ,‡Úõ> ¼­µ‰{Þòš;§ÁÓWÑôa(aó‡âr½nè�ª©¦ï@«>„ŽRs1`BOÕô» Á–]‹~ìÃ~]/¶ûÆYr[÷x‰�Î+$ý$¬î>‡1L’ëyÍžÃx�Pëm+^z7¼Jú/ ÅQ‡qáºí»AõðJÑô— h­eQð—m[4ˆ“¾9 ’þ3R`«¥"0É%gqâClJÑô )´¶ûêûÞK]ÊJvA`­Õê|ì«äVˆ^#œJêA˜H+º»‡ Ûó^Ñ»¨æHz	³ø.Œ{+Ëº	FqB•ô_a²mìB$rÝV'ýäœ¶rÛwÁè:xGHúû0Xw§Œ‡¬^ŠZ`”Ø ³ìœô£Æ½^êÒµ©ï{Ï»4fínÌu½tðIÿ$¬µ{‘ºîFà…,`ç†¾[­l—VÆ8G[6V?õ“ÇV[&<‚¨*TÒ?ÑÄX]îÜc­{Ý½F@Ò#h&$œv3ØJÞ4¨sâUÒw0«5�n
Žº3Oç‚’î‘�VJA×÷Ê¼X“aœc®åtfÓ„¶åeiŽc7EÙË«„ B éO@£ÕÍ5ßMªuÝ¶®EïÔ`¹k.öêÊ–÷êÍ«x€´ªÅn0ÔËNçBßnuÝá]×°’·B‰N¢9¬µ|–fÅUx¯éXkÍ¤âÊÖ*‡~ô†lŠ ª0¨JŠ01×rçº.¸V°+:ñKhÒõ>Ô¶–¢Â@OIÏ0![ËÎ»Y‚”¼CpAœŠ¦{ÐÐÐºâUî µ]l…£÷ð^ÓßÑH�åXâØrÉ>xç¡hFº
ËÁ|¼”[ÙœtÞ{ÀZÍ1v±j®u‡§‹•4ÃÐªQ¢zßZ|ïT%ý ‚FVšF§Ú
LsuŠ­9ö}ð–óÂ®Ÿ½iËûÆ¹—u[®R¤›ûˆÜ.ué�¢¹œóFÓ~pXeiÕ›7ß`"Tˆ¦ï‘¡¬E›ïB,mÍ­8ñQ`F¨BÒ„	Y=œ›Z´"ºEs„
LÔDŒ¡´bB—ýf5KT	 éÏ@#@1-*C'ÀÞJvAC "ÿûSŽÖBASE      1è  xÁA  ƒ°YãxÔ þµ,¹±.Xnlšâ¦a,µrYbŽU˜É1.ŒU˜É1.š+L�d�ÁÆ*L�d�e¦F²FŒ�‹Y…©‘¬cà"RVaj$kÄ¸h9¶Ø\·¶9� &cæ*Ö@—Œœ¶1¡A¬Zam¤ÛÔ¹éT·á\mP††3ZálVÐ´Z$Í`­¢&³Ú
�€	1I*˜TUb“2Vk«–&	-² #+,Ò€@ˆ ÈEcd,qŒ i.©&Î-WÄJ€d±ÐIaâ4q3…¹9lŒ–4‘9Ü(çŒŠcŒ	Q	ƒ&Í%TádYÛ
2f“d#‰\nËªÒÍdIN…Ù‡&iBPOS      î  xÁÁqTA 1ÃÅþóÔ³`(Šx¸�¡#ýý÷çËÛÛÛ×Ï×çç÷ßç¾ÞþxžÕû¯;Ï:çã9¯oö<Îym�Ë˜��¸‹së¤{”œÖºd—¸§Ü£j‹ÝVÑÜ“î‘ÚZ£Ë2
:©£°f·Ya&‰™E›€Q‡0Ì²dÆ¤ƒ˜Y0!03L€Ö6ÌÖpd°4–ÍÄ`2b0¶ÈÆ´ÉÈaÀš-#ä ‰E42ƒ†42˜`€4--È†5‚‚À4 ‹�"ÍBdµÈÉ’ÈHDB€Ôh12³�µeš63mÙ¢mÇ i3¬VÀ8Öš�MÎŒùóì/êCNF4       ôW  xÁÁJa†áwÔÃè�"Ø”"ŠËé"Zµû®±Kèº…¤]¶›�¢üÇ™Óó¯SÐsA¿( z]Eô‚Qw‹Ñõ!?~ß¥ßÃ|rN§”È¹âzaÁ~ã|ã-nn€›JÜhXhÀ‰©îi˜Q6,´¤aFÙ°ÐnšÒB_�^pSÍ®Öˆ]­'vµÖdÌTáæNRÂÚ¾J<�é0ÜÜÀpƒ¤–×ª3Ù¯þÀI`°_â©ý�†;Ð´l0üHïÍÉÓ|žP�çãœò‹ÞÚpÛÓa¥·O
(P @�
è�mÑnìTEXT       Ëô   xÁKŽ‚@Ð»”· ¨¼4BÂ 
JÛð‘ šôˆÄ90bzý}F?D ÷c„–¸žO#|}8ISK–œê‚9YjÑ2Ãuò$w¥Z¿D÷'©‘ßv«L*RV¡Ï›Žû“ÔÈo;I^±T¬¤¬‚ù6¶PVáLÍ‰™°PJÊ*œ©9ÉYXÑþÄ	]X­0^§ËT¬¡]þÌ½Þ¾ù†çtjRaýÂpi{XaŒÐø]p�ß÷82Ç¸ÇYÑ§À?>4¸CLIP       	           D<     D3®ZTR

SMP4      ?Î¦d  xÁÉ¯µ]zäß½š§öÞ§ß·º¯¾*7Èq™X‚¤\œ�“0  G‹ˆDBBÌ˜ r�ˆ%Àðo0@�Ä 	Š"ÙIUÊU_÷6çì³›§_kÝ?®Kþéÿ	„¿R�5RµMYïê]Û4‡nûðíâéñæð�7ßùöÓž¾ýù¿û›Ÿöýw¿ñëÿæo|þë?øáo~ïs ¿ÿø+¿eƒÃOÃðßøipØ‡?$T`(ðÓð?~
ü“üÿìÏ�/üù?ýçöñgÿâÿûÙ¿úÕ?ùð‹_ýòçßüüõ‹ùõË_ñõ×Ãp>¾\ž¯ã<,ë:ÏiKª*ÿôO`ÿàT„¡EH‚"E  (¢	H!¨"( B@` JP"TQ«¤
(0
@(ˆ¨…Q†Hð @ Ñà 
ˆB48PDE<DD!Y� BR ¤€á‡  ¤P(  Š
D(„ŠJð 4Ÿ#»Å2B«ÆÁ(I1ÆrÒ(YŒ‘à@!(¢@ Q	DEH„„J!EhdÍYÕ¢rÆªD&Í �8B‚€¨!Þ�BT JpRT@  ‚ÁÁ€¢PÐ¨�B‚b*BI	@1T5J€€¨ ÈíVh“]¡Æb3Sd«F
"„� R(Q� "šÍÀtôÑnq†ÍV
x „($Àp‚9§Dïê³Ð%#ÎyÃðcP¨	ÖŽŒKÒ*9°Hæ¬ôÆù¨kN]6áÛ TSÌô…u‘3X
L¡–%Ñ6Þš¸,F¦TcAr“HS”4M…³^œ@�“HÛJ±®çu3}Ó”Vã¨ÞÁx±Ö„GÈ)oÖV%lÜfØ‚^¬±-(PM³FSºÆÅT¥3Âpç­‚k‚««jš×£h•�§Ï†á3PÈ(Q}U¢Ë(Î;‡JÅ„¸åÍ”»]9¯çµ•ÊH4ÐÐ€DYŒéJ`›—ÂÞ˜,á[ DsÜŒÔUÓ<'¸Úyu†ÈKN¾ª�¦a‚t… "Ç‰uq¨äz™WëKïi4�°Åî!™ñÃi«ÚúÐa^ÏÛ°+�wÙ€á o› i`–ñá8£`x
4¯j‹Ö›43£÷%¬H†!Ö%£º�ÿôá½«ÛºH$Àð9Éº­…í:—Ë6UMQ@-4ü�·Õ”Í¡NËåçíònêì&’aB
�6ƒ²ó˜§9¢¯Œ�hx’·IíîAæñüvM•Å(×Uw§öt9KÙ–EÊ"Y°-jÚ;IËåbßzP«Ë%Öý÷÷xùâ—gÜô·÷�8~ýá—´�S � �¤z¸ë<Ÿ�bÕP b5ÆÙ÷mëâåtlLíû]Ôq8�¥i(QDC5 Î#ê»ï•ñW_|�ÝÓÁè"J	Ÿ� È:¯¶yS!~út-wm•’UJø	H#yÔíæéPàøõÕÞv¢YÁÐ€Íëæªn—òe[Ê²(¡F4¼iLÌw‡ûe8¿ÿÚìËÈ ‘G˜fwØÖ×upUY† Ò™æöMß¼ÿøü*m·Ë‰ª"À×òígÀ—_}}Fuw³+�ã‡~ß«fÃ#Ô€1&¹í{;¾^ÆÜÕ¥B•�"ºî¾uÀùWqÂþ©0«5Ñ˜ÖªèMZ§q-ëÚÓQÂ¯�4’æ³´OwîÃùËW·Æ*¿‚"sÊææñPŽ/Ÿ²¿Û•T€¡!š—-W}�¦ø²ºª„O”ðÀuJ]ûn]�ÏŸˆÝÎ"
…\ííŽry9Åf_ 
ùœ°ÿv[|8~ñpWI²Pá|Tó�7È¿øâýO‡›}�âùëK¬n[$0<€ tØÜ<ÎÃñù(»¾ñÉ � �ä	lÞÔ8¯ZûV@i—d»²JÛ´jk�Ï”ðC ä%òð¶âñÓ‡lïzUÉ0á¯‚ ÈgW½©púxŠjvÎl"&T D·Òìêê:*S€F4¼p�R×?º—ëñ±Ûy…ÍBÎÆìnnÈÓË)ûÚ¸M@
ÓP÷}•.§ÓsÑÔU6Y	‘¸è?;´Ã7?¿ìdWrZ^Ï¯å¾u’¬RÂ=¶-ÖÝÃ�¹_V�ñÉ€‚´ˆ«î÷ñzyM¾¬%›L†GP ®›q»=Öí<¥²* †BÅj6{xhÖ—ßŒ|×)¥„ƒ `ÎK{×öøé:ß6( †óÒÔ¿,—ÅØºÉFÁðË˜ÚÝ›ôrýøZÈ}S(¡bSZÐßí§qJ�k@“aÔp‹[Ñ:_N±èw6*Æëên¾{ƒ�_ýêŒ»æö¦†÷×ußï$`x AÁ2mÝm]Óû)w»Öç0üH‘<l±{óíözüÕ¸<<te„UJx€¨ÑU�‡m=¾L¦­*ÚH†¿	ÒHŒª›*^®¯R6=\þMÎŠîéPNÏç­h÷ ¡ cZ«ª®¶m<çì¥ë\=óÉ§²hZ ŽëõÅÝñÓÇWîîÇ`h@ˆ¦ÉhÓCÓ�&©šdŠÚM_S~÷C‡¿øðt[KZ”ð€4’¦Õô·�<_¾9KýÆzRÁðÒ˜§m÷Ðštút��©+?½<¿DÝ×Ž~
 k‚9XngSÖ ëy²÷ß}À/¾úÙ€w�}Z0ü!H]Ç$oßVzüæãˆ‡¾IPÉ0¡p²¹:Ü«Lï§Q|_÷Ï—WãÛFV2| æ­hÊf�¯M]	@Ñ�=tŒãyPVÕn×ãp6Í¾‘(PÉ[^ºÝNÒõ2©-¼Ë† ‚"ë´áöÝn?~õbî*“I€Û*UuC?ŸÏÇîP¸!Ãï€ `›g½{S\?~úò„§¾Y2"TPrŠ›¼­ñõ§¹,v5£RÂ�AˆÆÙå4ÓÜšÊˆ:e-oüüúþÕô‡€ ÃÔ€yZµ}zìòËW¯ö·{¸×ó‡­}l«b³ Ãg D¹M,noÜ8�ƒvÆdx @\sYÝÞN|~™,ª�
„ßÂ4$çžöñ2œ"Š¶`r¢áG`pˆó„îÛ·xþâ/^ÊÃÝm1m@Œjýí>§óGÄfW“€„?äydó„a|]šêþ°Ã4Ÿ.¯Õ®…Q#þC¢i4æöÐ§õõq(Qˆ†ˆêâM·³Ë|ž-«ÚÓQÂO@�`‹m×¤a¼&¿o›}�¶åõZ»®±�Ð€Õ9çzÿ€iùt¹4µošÆ.ë5_v†E×¹¼^×d�Q¡„¿	B˜·IêýCÑ<Ÿ>NØwÎ¦,¢áû ˆê×C½3¸^gçlí²ŸƒA\6ÔïÞÙÓÇ¯^×¶ß—*Š¼®¦êïRZNg½v×u›Ž§KY6	Hø¢œ%·í“ÍóË³š]g@·œ]½�y½(vÝ=^Ç�£À×Í=˜——eÛÂ08pÛ õýaÛ.Ã•£oÚ½ÝÖñãºU½«ÚJ†ùeZËƒwJÑÐ‚ÍëdÝÍ.çéøº\*w×–Õ²W­|Ël‚C^FÍ;`}}YøÔš²ˆ†Ñ<ÓÝm\.ç+«}aM†hh@MóêÊ}«:+Ê²€Qˆ2¯‹3»ëz¾ÂÊ®;ôåy>NìlµïíuþúËMö;'Ñˆ†ÏAˆ¦ÑÚ¶ÝOz™†¶v…Í€1ÎU±«1�ËR¢uw5Ïñ|ÎVŠ¢ïvK<ƒ­öÀà�Ò‚îð þz<ŽYê¾=D?^/ª®Ýµ-®ÓµF[ZE44 @¸Í´»]·žO—­(ëJ-©áAÕeE³ßyŽ—©ôu“,5|Š¨®›ñ}[¦ë4n¦ªÅGˆ†ÏA‘¼­jª›®NÇÓÚT�…�þÑ4¯ÞJÈ5Ž±è«C·Éõ<^<«jo=—ËUµ*�d� ˆæu-Ü®Ç2Ÿ§èª¢ ˆhhA‘MSô7µãxžëú¾é�¦vEµz£á	Ñ4Ï¦Ýß&]Î“w¥/˜�hh@‘œ&xwCÝ¦ó¨²/°ž@MXÞÜÉz}y9ãMÙg¸¥Õ¶uïtý´e5»®õåt.6jo( rœP7e·¦i]Ùõn‰×!±©­±9Œ¤UQßÕu¹Ÿ¯À®¹iL^×m&ª¢ib¾n£i«Î·i¾Î@ïšìˆ³ñ÷½ÚËñÄ®-Kf Ôu±èö–Ãu‰UÕ:“h�F±U¿kÓðqpwm]&
€œ“ÛngóåzmXV" t‹B#šÆó˜DúÃ®Î¸œ6µ¯©jŒ2ÆXºÇÒ�—ókN}Ûu®B–µï”ÖjxE4nY»»R–étþ�»²–«¡E4-1u“uJí‹ìU4<�"Ó,îvßäËðø]¹se^fM´…sbDCŠ0¯+ªò¦_¦ë8³Ûu	i4Z?ôé2œ>�®îº}“ŠåzQùÂXjÞòVV»"oC\é¼·¢&´  I›jÙ>€ÛõøL[·%–¼Žc\]Ñwµ¬ã1F×V4¬[òåm‡óø<Å­Ý÷]WŽËuvèªÒ¨@²nI][×Èë%/¨Ë]QÎãeÛr_x˜,BÝ6±u·Ãu~9E[ú Eò—Æß¹Ž—ê]_·….ã¬±3m25(B�q´n»ßÖÓõê°ó5áDÃ¯�y]ˆò¾vÓñ|¼V®¯œŠ	(Œ×~ÿ¶“ãéÃ	Ø75@Þ‚y]MUµM¥—×SvMS·y#R]µ;-§4Ž¹¨/b4üT—Õ¢ª*,ù8­¨ØAÜÆTKcšÛ0/RÔ]_»yxM£”¦Ÿ$¼±qV[Ô>q‰9»Jêä¬†·Ð´F/MWIÚ.‘t®®\–u™#]ÙÕµ,ñ8­bvûƒ™†Ët²(
cÕƒˆóJx[ëë—óV<Õ=$t‹ÖTmer<Ž„+Ûà2ÍIKÓB¬	=¨›f)‹VS:o�Ò[G	¿ê–³”eKLËK8gaÃï‚Øòä‹î¶ïòrüˆµ;ÔN­�`Š3YW54OÈÎ–°*á?sZ"M[ÛbØ.k’ÚJcT·”lÝÕ~N/ËìËÒxäðëÈyMÆ´�Ež%kŠ
EÔ´Rµ-›VÓ6-º ©û
iY�g+õÉBCÕ˜Á¢qã-ÔgÂ[�ã–|ß–\—ëlsg½±j©QçšÚÇ”´ïÔ˜ð Pæ9Qª®*8œ#c]:çàDÃÁ”Öìl[Š™–AŒ7¶°4&¼sŒY}ÛD“§­«ME!5‚(¥J¢#6ß]U§8%J#Ö³P	˜sLÆÖÞÚmÅ¦ÖXþ²FB_ZÕ%G±…bDÂ@‚iÙXÚ}wXÖëóÂÎÞCŒ$ü%03IBáJ1y]`\Q8kR�)Ä¸‚Æ„Ÿ€ÈÈÌ‹×¾«k½^®Þ{SÃµîAj^’úºõÛuYà]I/ ¤œg'»CÓÎëùâ«º4ErÎ+áß IÍi%\Ñš"­i€©\ã¼‚Ô•Ó&ð0(i%¼…æ´Ñx_p‹igÅ‰jNt¦Î>ÆU\rÖ‰µá	„*#5»ªl
NcL¹(MiáÅ˜ph^7”r×>]çÑØVjÀ9cÂ¿"‹æWc+[å¼^“ñpÆÐŠ	7P(²¦dLSvMÓ&%¬5�‰SUÔb¯ë:©´MS9æe\!¾*�*×�Z�¨dŒÖù›]¿Œ¯×sª+é©°dL1»¾v×ùÇª)
:hŒ³5Ý®�ötz�¶ÜU“7)Á�H²RUÎ¥m›*_•fãœi�…7šæDßµÎmã6Fç]i(áÛ @u“œ½÷U²²¯¤õ€®+PQ
Qly³Uyè1Äã8ã¶{Ð&ÅuÙœÍ%½Xc$<@™óêLQÁ˜mËØ %¼	¿B©9GAé Ž9UYz2/b}¶(Õ˜ð]ªy#rUFÓÁRñ°�p"kŽ›5m!šÒS8k½µ™[Š¢¼{X××ã3¦®*�Ðc-”J%T·tMé¶õ:xúÂ§þ:É	˜Óê«ª`Ì_WÒ~D’[QVÞÆmYb©­ïæ-¯™–â=�¥	· G]%IÕx·å™�ñ¥ˆ¿ªP5¶´ˆËê¢qŠª
ÌÎ›bÕeE‘�±Ž"€* „‘c×—&�ó6ª…u– D  F©ÑŠ+Áy6E‚·ÎŠ‘ÈM+_;Mq¤xç¼‡À°‚
… šmQ@cTu–6Óz�Å(¢SkœÏL“E‚14~D†QhÚDj[@ó†$ÆWðI×¼5Y¼X!�„¿…ãoý¿ûœA‚ !jFA€€R(B!) �Q jB$ bD¬@„¡ªfe
!‚@”@”BB( Di$‹@Q …B
%8
H€ÁA@
)Tœ€"
*ŒGÍRQ�1Œ1@T„¡E(<DET D  (ÁA R²2‘jÕÃ9¤¼A�/œÍKŽš1€B¼1Î$Æœ	+…Ï¢fŠca‚ƒ¨!EE(
€ *sk|�"¦-úÍ¸ÂŠOyÛL¤ÒÑRSÌ(\a7·ñpž4€�B•Pƒ)T(Ì92KUˆË7 9ï=­¦ÍgëjUÓìÔŠë Š…¨€"�9­ç¸ˆÙª±N<½
T#WkêÂHä:–Rq !T jÞ�Õ—¾LHq!`Ea]ãhcœ`˜Ä[gÔ”6�m
ø„	„fk-B� ¡hh@B4|
³¦¨Þ;±@’X8g„Ð7 öb‘Jº²* :G…øÊ9�ë¬^:�axÁœRô®öJ�ÔnÖ:8Ëð' ˜sŽÞ{gbJ«ËÎ8Ëð¿�`N1ª«P%�‡BÄxç“š®Z×míNÓp)roêªóË<QÐ×Í\ÇkÒCáE á-(@ÂTº®l†u£m\­	ÿ 2iÑ´&Ãä¬/­!þ2 jRZ�Óe�sE‘­Hxšg‰u³÷Ûðº‰õ®¤5`ø b,Š¾/Òpœ7kŠ‚Ùó2›²¿Åúéü2jUÔ¾÷.ÓP:ïv»Ê×1Ùºñb†7 €<�wí~½^Ç¥jc3Àð_ƒ bžŠb×óéºõûÊGþW�¼Î¨û'_O×å¶ß	PÁ:ÂîßÁ¼?}µ Û»æævKŸ†£¯·¿yÏ¯Ÿ®ËSÓ@U2Lxi$O€¿©bº~@.»‚YÀð�@‰«øî¶,_ÇWø¶…Èð08¬k*Ú;™æÓë28cû{›çóuiöUMÉð¨ã&¾½=ŒËé˜§¦5F P]Ö·oìõòþY¶­nÝ<¯[å«²»A:^ÞÃì;OO �¤)™òvŸ¦ÓÚµEÀðA@rP5‡Þl¯‹¯û²\=Èð9H#ºŒ[·{:œŸ?~šö7 C5`ÚonvãñtšÊ¶­%¥„ƒ€ä8Ðw‡nX�›ÚÎÐA)@³¶÷7»ôáø«ˆÆ¶}…ñõt�JçúÃ>Ÿ^/CÙÕ•(•A \gm÷ûŒãë(e_TJøc@^&ëªey¾\uçk&«”ðc�Fò6�7ž/×e­‹TÜtÍ|y¶Û®õÉ?p�s³?$ÌT7¾\-Hâ¤¾ýü‡çoR³k�4¿«Ë•»)Ýò:|‚ì[K�  Æ•e}Ãòãéš·¾’H€á�AI£ñM_Ìñúj×®*’€?�h’iovñr¾L®mŒf€áï€ 8›­ïzœ–Óà*Wm>“áG  )/°7}Ç×é|Ú•Ì.ª„$ñjì¡“8ž®¾,K«†  Ë,»»®�çã)ÚÒt7oæáõtívUcW0üû  y‰S}84Ûp¾T¾¨
†€ ò¼Úzßæm£Ö¥5 €yØÊòé§¯Ã¡é<²QJøOA :ƒ‡~¿Åa�Zéoæ—×ç³CÝ©¨d˜Pƒ€è¶ÒÕ}Ïùuˆèš‚4¢áû  ®óZ÷OvšŸ_¢ëv.¤HÞ­Ú{ÌÃëq¾ØÊÞ>êøåóóX´D2ü@—Ùtý.oÓñlÛºb0|‚"Ûº™ûûoaº|œ=ª²{ ^NÏ×T•ÚDƒ4mØ?Þ›óËËdMmD† ³JwßçËŒæÆùMH�sîï¸^žO¦é·X�á' (˜ÓZÞÂ=¿¿àþ¶óÈ Ão�€$}µ¿Áxýô:6}-B†Ÿ€€$�Øìz]>½@×ÝT˜.f‡ÖEž@�pÛ¬íZk®ÃÄ¾~Ó68ó
éê}]ñÓé4bßT`Âj^FSìob?ØS_7€‚¡!šÖµ,ê:¦ë4@¹Û?ì¶øéý•ïš"Ón“Úýçñ|<[ë›þ�Šùåõc½öíÍ›„çç�“éwÝ]³M§�×è÷�X2|’·Ñºæ	ÛpyŽè§&�3«ÃCž†çgÓV½M°ÔÐ€ tNhn+Ì—gñ]ï’PÂßè¨©½¿9\ŽïŸQíoÀ2Ô  ]ßÞÜWãåÃ€\6õþéfúøþ¤»›( CH«˜ýaŸ–—a¨‹Ö{ZÐ@²N¾»ÝórþpÊ¾wQ(áïƒ€¤Mã~ÿÔ¯/_s×öHBØœ.âo»íú|ÚŠ¦­½*ÀÐ€Á–F×Úõ2^žÍÎWP'þ ×(Úö–Ë:·(ñÐvn¸‘šÚ
3þ „¨ÎI‹›½ÁËËIPwÙˆ†G¢ip±ÞUrçëÜ”¥KV4ü„hš2Û‡=ÍixÁ}Û»ÍQÂç „9ÎÉnnçùô2Ù]U29Ñð@�`K£oöå¶�®¹lËZAÑÐ€áºJÝìûbºL®mÊ2Ù$á‚M#òn_—�¯—}Óšl“„?Ek\›âÆÄ4Ì›oªj+T4ü„0o|³ßÏË0™².­KV44 @â]÷ØÏç�¯¹­¯Nè¶±(öq¸œ²ìŠÆ �ð{ @¸®êÚ{“òøþã¼o[áj@Ý6)v]½¬Ã°ž›ªV¡høCPDÓ²¸b·/§áåÒ mT`5üÂ-�©¿¹í9]Ž¯VÚÒF“ÉmÓª¾ióeüˆ©¾ém6IBŠPÓhŠnï‹mFSíJ+� „1�¨»·uµ¾ž¶ö¡jaDÃÿ
Ëœýãîa¾^?|µöÍî®±iž^‡äÊÆDC ˆæeú›®|½^¢k[§¢F�™Êæ©2éåÃ¶TuçMèAæe•¢ïë¼^.ëKê›"jø ˆ¦yD»»³ëåtú›]-QŒ†Ñ¸mhnnwçëóñUÑ”.«ˆ†ßE4¯«sÝÎÅËuœMë=¬Š"ü6˜ÖÙH}ßÕ—åetöÐÑ*À˜âæ«Fb\¶1¦2¤„ÿÑ·Xt]¹N×ë¢…7e6&üG .imË¾¶i=ÎjïŠhLø/AÝÒR–}-2Ž×Iœ/À†ÿ9-³5]ß­Óë8Ÿ\]”*áïBuÙ6©»Nu¢ji*—œeJK‚«k‹<-›6®ò „ªNnõ~ï;3¿[á|éa•á-¨KÜ¼Ý7X—KÜœ+m		=˜â¼®î(ëzÌtâ½ˆ‘ðïBÓ$›t;g×åtiRë[U/Ô[ÖmŸ9œÏŸÊ²ôÞfˆ¦4ÒVÒ2ª¥©ÎyKØWåœÆ:ïœ	ï º.Ž®ï+3—¹s¦´bþLqòÑµ•cœt¡…7(T$ü6¨1mÎ´E�K:¥‰�«�‘ð—Á¼i‚m[ƒež×ÜHÕ5eÓ•ÍËi[mã+1á-«:ß”eÚæMÚù¦S³nÓá¥,’7 ¦9Sš®à¶]°ÕÆy-~Lq‹0­¯—Q³^
›Å„'�‘Æu…IË��)P¨PcÊ,Š^%-çuCe+�*·ŒlºÒ›y½Dª¯›ÖD®º±@)– r±]�x�‡YÐVt"æjšÒAÓ”œ·Ö8þu™yÖ¨�/E·‘,\a­HŽ[R[UUÉù2.*]ÝD“·mu¨`*±F$ÜCH²!»²ö²­WMEBR±Ee]ŽSR­a'¡�
4­$«ª†ÎË”]‰ÊÂYHøC(TcFrEYš¸\¹9×=Ý²¤¬0Ö‹³~*"A]z—Ò²I	©Pd‘œWF[6•,ë¸äZ¬3ð¡©šVAQ”.¦u€©`Z�„DfÒ-‹Æbâ,Î¹ÂÂ$üH™—lØ-¸\5¹RJkÔ 9®`Q•¢qX3+Rd1ªQ®ñ•ÎK„ŠqF¬…@ÂÿB©)—¦ð	£*”…81&üu¨�šVlµm¸Fd'b 	ÿ%4mÈ…oÄ-Ë5£tÞ‹	w B�ÄMLSxÉÛ¼™")³M�PA�„˜Œm]Å5�ÂR¢Ì&ü>H(ac
`š7µ^¼1¨+ÖÂ¶E¡k\•q…ºH¸ºÉ4¾²y�×,¥Žb$ìA2ÓHÞ�Ðº¹RjëJ8˜pU›É®6iI…w…ÉB$ü¨„2oYL]5š—iÉ®2…8�H¸…‚º™(Ö×).+
kJ±�P€”Dnð®-©qŠpÖz�¡ ’@Î›d_{‡m^½w
$ü&‚§¦êkq9ÏIsMOŸƒ ÷ D	*È(›+ŠÆ2�\
S°€³j  Á”W´®¶y™–\ªT¢" Ã=€dQ]}ç¤eØ Þxk`Ã�@d€ÔŠÊ›—iSSU)ØæœQÓ”pVÅ@B‚Y5:ë]¡I§r«ÔÂÂªæM)UimLCŠâ­7*öP03/È¾ìœ¤%CÅ:+Ö!©€¨¦Òx+)o™^¬xX
es¶)Ém^E¹ƒ¬Ó"Æ·MG³1Š+PÂ	�ˆ
a‚!* ™l¥t.IŽ³q)²Í"¥„$`DÀhsvR«Iy¶jEHÆýWéøßþ†ÿÅ‡’*a¬P•0NÀL¥ÐˆhN9ÓX¨*B—Ä€€ÐPTD©P(‚€�U	X±&S£5€ (†*¢ DÅ$…�BŠŠ�"
¤ ˆ5J!A¨Š( „BPÀL¥
D`h¢€&8(ƒƒ(¡(†bÒ B‚T�€Æ¨Q�
@Q14 d� ˆ	NLYI
@�ƒƒhð CÑPA�ØN† YÁlÄz)2SZœŠ+ë¢ÙÒ–D«è])ÎJp )¨�¢J¸ƒƒ€HÉT¥1Þ‰$MŽÎÐfHØ�"* 
€ iHQ ¤ „  
!!dp „
M™ÆRR°aCÌ"ÆŠÃàA B� @�!Áà  EiÂ-(B@4 \MÌµoá×mÂªuvÅ´›„9k´Æ×p°1§µIbŒ	ÿ¦¼®(lÊsŽŽÞ–´ 0mªtµ¯#–ˆì�/�„GÌia–¶5’·‘Ög¬€›Ä´Þû]kÏËiD•n–m·„GPˆ,‹&_Õb¦q�ÎyïiÄ„GPÈ¼b-|W˜ÏP–Æ±†¡šr„Ô¾ÐW15­W—)ª«Føº.†4TçK¯e2

�×­dÓ6Çë,¥+(Ž @Óh|qèÕÇª}]$£`x!™ë"•¿oóz91¹¶DºQ][•\§ÕxxcÔ„'®ËÅìÞüZƒŸù«±íwÝ¦4 „{ ·U«òn·mãÇ-•·e­VÁÐ‚ b^¤½éo–ËéºI[44ÃÿBT—õmá®Ãó'­ûZ¸
@€Ëåá‘Ãåãéêû¾ŠjÀð‡¸Fwóð-ŽÏSÓ?8—(ª›s»Þ¤ø2;[yC#AÂH<]óç¿ö9ðgÿü—ÿøîé¶ž2 bÂoƒ¤ÍJÙ˜§Ùds¡ûÞwÞàùg?ÿß½oLTBÅæee³»Ãx:×Û¾ƒÉ"þ$¯“µ·wîøòüzÚ×;£„R€<¤Ø¾{ûñùÚC‰
†08äë%6ïÞ5¯ÿåË¼ÿÁÁ/Q”BAŒÚºã)¯®p^�hx…‚¸M¬Þíñúþå}¾Ù7NA³9î@Pd[g_ÿÍxú‹o>±;ìgDÃç  y}ÕïÌôáåelë^2„

€u[«]+óe¼Â�¥¸-+oæãÇ÷‚»¶JÙ(%<@Ái^ä;ß=àý/~öŒw²å¬
Ól¤mŠ¨ç”«ÂùlDÃ#TD0_Çê³_ÿžö/€o½)ç�JHøÛ @Ñù¼â³·�øâßTøö�cÊ
†ÿÀm2Uûdçñå¢}Õ{%5ˆ+PÝ¶y9ß7{ße«J	� Dã†²¼±²\®�®²P
…àš¨{+ºŒ‰…·F á¤@Òp]¿÷ƒ§áÏþÙ/á>;Tš `øÛ  Ðx^åé;}üæ«/_›öÐÎ‰ÙlÎ„ `œ²<ÜWqyyŽ¾i=" 0ˆ£¸‡û>úøáâoö•."&Üƒeš,º»u»ƒk\¯P
a5ÇÍU½sq[64DÃ#¨º�¦êé_ûøó?Ï§o·Å–•�pR$ÍcÜ}ûPáÃ7/¸?€¤„„ä¸¨k[ÑuÞlij:‚ òu3Í»¶¾�Ÿb]£z{8ÌÏ¿l{Ó‰¢”P�4¢sÜÌ·¾Õ�¿úðÕåÍ¾ñí›§þõù—ìo{CJ¦ ëš‹î¶³çã	õ®#
‘8ÏkÿÝ7÷xÿÍ×¸hW%Ü�BA·Ø>¼mæO_|½tµ«¤Ý�úreî¼÷
»Yƒåz-î?»Ë—/þâÈoíjÛn·a¸NMÑ¸¶¿1ãõý'SU�d!pYPßîºóõx=Õ·…Š‚áï�MËÚúÅ6DSÑÐ‚ bšÐÜTñr}…ÔEM`Ì±(újÝÆqÓ_­Þ€´Y†hë»ƒ[�Ç5v•a0|HñjüíMu~=e<T•ÝÄ†:,Ú½Û»ôÍ—ãÚ?5•&‚áû  ]§ÜÝÞð<|Z–ÛC-I†¬C¶·Ou9}ºNMw‰ Ã ­^»˜Ç«E[»ä¡á	¤@§y«¿×]¯¿|iš7{k¤„×D_9	  ¯›+û:­Ã²ä®°†”ðÿ€4¢cÔòé¾5Ó×·ýí�W¨RÂ‡«9ÜÝÊÇã{bçÊ²¾­ãô:-í®p«‡†€ tÙîÍåúa¥´ÅÃÓ—ãWk³¯û®‘y>_FVU%É€á	�cÛ]·,×ã	mQ'“liòåmÍù5N¥iØÖ»œ^§WteY.Z —Á7ý#æËË§\õµpÑð}P Ü5»ÛÛm<_—²ô©nïí|yÿêëÒµÕ­,Ãë)—m/f3¢¡ƒCZÖ¸û½•ŸþÕu}ÚÝÒ
�‹:úNÆÓÉ}Q0üUP ¤yÖÝÛïTÃ§_}½UWù�÷é¸LÞVÞi–ðÇ  yäRÜ¾{ë>~ú4¶¶i2|
€|ÝÐ¾¹k/¿8/Å¾7*FiÀ)jyû½þã§÷`û;S»Ã|œ7ëÚd(>!ãäŠ]SÌ×óÖï|�iDCLCô»wOýøüÍuÝ=4Ž2üH#iŒð÷·Mqy¿\êC_eŠd˜ð—@ :å¹<¼¹)>~úî¡©�²ˆ†¿B4/ƒoÿÿ‚àœ×¶5;ð;Æ×Ífu{ïÓÜ*#äºn$#d
(Û‚1‚À¨"$d‰DBHÈ‚™#ñgøˆ ãò=÷œÝ¬µ×Z³ùšñò<OÉ_ççëM�ýP•
ÚÂ”ÇPïoWSRQ±éßƒ€´r¡>~:ÊíåË½Ÿz�9Úô —¼…ÓO{û‹·¯÷0únÊ4€"fÛæºý!"¿_ËàS€Ò¦
BXì†nŒÛÛõ¶¾sã¡Ï6½>ï‡=)Ó¿¨[´}Úå:Ï3zßåPeú9(R8»”N�ò^KØw�ÎÙ´�"ÖÖlxˆ¾®×šƒï¼›þ(bXpÇé§ûãíùëÿáÓn«+›þ­lM»Ô¡æw”èœS›FP€¼¿ûü�ß|ÇîiZIêšëðñ©×ó¯Ø¸?…ÇáÔnËÛ¥öû˜Xýô—ë†ÓOóýù/ïËþãÑ¡:gÓÿ El+wœŸºõövÎ5õ#T8ýO°æJí#°.™�OêÛô¿@+µ…!u­æ+ÐK¸é‚"f‹,CŒk¹½­Æ>8sÓo€VèG/›å,BPÊt[6‘¡óRæ¦è59Êôû ˆIcæ.>k>Ï«¤Dtúß 37u½ÈëR%Ò5‘é;�uóË¡;�év»/1¥ÎWß ¶¶5È>u²äK‘A“*eús´š+ÜúÊ{)ìT)ÓïÀêº7Nx}»:t¡+
�þÌ2šöžäJ‚N¿ÑòæÄ�ŽÃp¿.ïècT5:‚­m´Ð©Ö6£HTŸ¨:ý#Ð¶’[ÚwÝ¶Í÷EÃÑ%h›þ „¡röcj\®u†]l¡hÙš�CÝ¶bð­‹>ú²–‹:Ÿ$8“iZË¨ÎõÊÚêU³H„÷:ý-¥yÝ«GÝn®EŠ Ó¿‚ióÌlÒu‚ÚnD'^!dµ’%�=Öíš×–\Þqú3Í¶J„C-ßÖ"}’ŽÎCtú4Û¬jû Û½‰>yQ•éw`Í6•�‚¯˜Å8ñÎMŸA˜ÙÊìãØ{”ÛÒZDR…L¿€ÁÈš�ê‡Î°ÝkÛœ…~PÅÌÖØÉØEWlÖ˜G4(h…)©aË¨ê#T§?ÑJ©ˆ!øb˜à)ZÆ2ø½ïJ]ófa]HÍß3›K¡ [Ñ‡àK)&ÑiñbÓ¦­.µ!ŽI¶z_oªct^M´š�Æ¾†Z7ŸÍœ“©i`ÉUÐ…(­ÝkÑEt¦àôO@­Ô8Š¼Ü_“&¯&‘é¯�¤�­¶’\'lµ´æEB€ d«•Ú‡ ¬›øªÎyï(�<&OÕ`{�Ô¹TÅ¡ß7‡åÖ$:)ŠN¿	’ÍH�’ˆj•MDEÄÀ¬Y\ˆ^k»6ª/ªÐé�ÑJYkÂ¡ŽßÂYè‚OnOeúÂj«)¹¸Ú¶º
Dª*ˆ*•>¸Ð¸e)ªê!XmÅ…à<Ë–}:ç=`,Ò˜Bˆgs[Rçƒ¨Lÿ&jh5;F`œ)œW!dz aäV[‡þ€z»,†Ð9uMT§#H¬n%aìƒ_ÛûRÙiòP³V[‹€º•5(Õy…Lÿ¤4¶Rà:U´’-ˆ©ó"•©�ïcó¶Ö
ŠS¦Ó¿M(f–¥9—:až×ß¥1\Ë{»3¦ÝhÈùÖÌ‰Gl0…èôÇ š×i@nµ4¥§
(2E˜ fY*ãà$Û\ETƒSETDlúH@kÅÔu¡GË·;ãx<(ê½TITIš8ŠÊô' ¥™5À;ïØr56ñ L§‚&&­9ë¨äZ¶dš|R¿mï­Ž:ªu5C×<"•¤dúç ABZ©Í Ü[aL»®Gi×V¼‹ÞYMTE§�b @ÒœxÖÕXDU@˜Rtúm&BZmÐ[(¹P‚©O�èô=H6+.`$WÛ4 b" L„„´VÌ\�o[Î-v]Ü†›‰‡“ Þ@« yD­–—ÿ¤|ùoú_!´é7A�Å H! ekŒ.9ZÞ´+ˆ
GT/T%A€ EÅ(‚ Lb2u @Ä„€@)P¨@(Ð¬B(Bˆ‰Ph€@c36@¼JPÖ’­‰Õ‰:è4€ •D @Ä B�"@L B   $„0T5ƒ‰R”LžV­5sH>0°mÜº §øª 0ˆA
!6yb"’€"Ä¦ R!J*­d1„¨
H®”D ofµ˜º‘ÚJÍR`\P IXkÖL}‡ iœµ6'žÞtêÀÉCˆÉÓjÙ :"AJ¾•ªÎõ©Gªmnkm‘ý.D—[.Šà\TQ("€�f­(4u‚:»+|ð@EvÜ ‡µÞ
ÜØ�Þù9¯k�Q! E‚&°ÚVPÇÐAs]‚Ó$^¤@H!H1€"$ !HP¶,Íë E««Eô]ê|m·µYGßËÜÞ½:çÅ;Ç)‚‚Æl@J>f{qAÊé(dÛP-Åàs[²×^C¢€–IÆ.yÙ¨�— ¯œ>�`kE5ùÜf šzuÂiaV7Aì²–«QMtúZ­ÕBð®b¥o"Á+ VkVéFßd3Ò…D…Ó/A�YÝ¼%õhu®Š”º¡ø²¼×l;Ý¥(­][ShDpÊ©ÁVWæØ§Øl®!/Îqú	°Ô¬i|èÚûõÎÐjp i\8íƒÝÏs'Cìª9Øô©X­v}/Û’MG×"ÓP¸%wìÂr›k:Äˆ¦°éW  ­ÌÞõûàæë½r—¼R@0·5ÆÓØo—ùìÓ1*¡àôG PX²Ééð±Í—óWç´C§Ï—·{×Eì‡ù~~[÷}ì©§X—»ô§G¿¼>ki¾‚Óg ëª’[¾¼Ï5ž:sBa�›?~ÀùÛó9í÷^©F™>ƒ€+ÚGl÷í–ÜèÔTlA \š��}½>/�qÀéW €ÚV×=(æåVÃ!¥ìa`AKÇ',ï?žm<ê*Àé— UêÜ�K~;gA'ãwO¨Ï—_ë8¸ÇÃçáz>ßý‡G/4€Sàv«Ãã§Çúòò<�ûc@£LŸAªØú.»‡Oë×¿Z·ÝÃà	B »7¿ûî$—óë•�O½«4pú¨u	iÜK{¿RÇÑ©Q¦LÁRª÷¸}}ÿj]× àô+@­sç|ØÎóOƒƒ ‰J±;�úöþcÃ§nG1iÐéA \Wï�§ØÎo7z„ðÝGw{ûáÎ.¸qÿa_o/?ÚpÚE) §y]üþó�Ë�oóþ¸ïjuF™>� ¥®ÕõŸ»öòú­è¡Ä”TpmùððÑßÎÏ?îFgàô	 %{Žû´Íç+œŽMœF ÃxúXq}}McêuiÐéOA@Z¹K?ž:[^¯HãH3ÂÛZÝî£_^¾Ö¡ßE)Ò tÍf†ÇÖÛ·ëÍï;ßpú¤òêÜ±ç¶]æ-¢?¶õÛÛ_¬]LáôÐßÏ¯_ýþ©ó� §À<«<—ÛÛÙÒxðÔBNŸA ÜnHN¡Í_íNÉ5¤ÖÍÒxüÐ¶¯¯—pÕŒ2}´YÛ>vXç;Ñ÷ÞœF�¶nèŽ\¯—çÞïzÀ¤A§?)¸zÝw®Üò,]ˆ0%6“‡‡q|9ÿ°yô½Û)°%søxX×o—÷~|Œ¡(Àé·@ˆ!g‘q�v½¯¨"ñôþùë�švn7<Ùöòú–Ýnè›€œz�¶ÎŽŸ†í‡ó›:ßpúS°n@÷0àÛõõÇ6ÔÌg•ññ˜ñ|¿–ðèb1iÐé#Havý¡wëºÎ¡OðF™FbØ6v;¹¿_cÿLtúS�*uFð�¡Í·[õãèÄ`¢´+uÿññ=¿Ý–~LIªk ¹ÌÝpHå~»t~Ÿh”éç €RsÛ�a[Ï¥¥Sp0pú9 Ô-Úü¾DŒP£*Ùr�AÖ«Õäc0/6ý6)Ìyü´3\Þ³ÿ®Z(6ý	j«KM�¸¿½½Èî¡‡©àÚjz<Êåúöö£À Nÿ&ÎÚ½„‡ïúÝÛùù[Üb�2  È5ûÞa½­kK­ûpÐ÷·çœ¦n×ámû²j|ò4eêA�KÆ¾®íuÝBŠb
NB¬.ñXýz]²îˆ›¾©ÒæªéÓ§~ûá‡‹?^Š�Óo�T)}ÿa—çÛyÛÅ�²*8ý}�®é�rúô€óËyûÇÏ÷åùåžö�)œþ€Ûœ‡ãOëuûñ|�1¹Ýç�8?¿]ü©�ãñ§-¿~]oÚ§ãñ¡./ß²|<D ‘T”²…®ïËv/:ÍQŒ"µm2ž÷Ë[	²Ÿâîþõí.‡ýÚ X½„n|B~;?/ëxÝDlêAˆaEîvOn½]ÎÒ´óÇ{n¯/%¤ÞKf2}‚)X·¢‡Ÿü$Í/¯/ëé8šU€1ý„X]×Þ? e]6·ïÔ	e@ªÔ;¹ÿ´w×ë—ÙÙ~'Pa³´ÇÓƒ\®_¿¸ýiT5€Ó÷  mY[wz�ûåÛÛ[‡Ø=Ýüã·oð^Œ
®†áôTÃÛùe}Øõ’=eúÒòÝ¹Óæëùõn§QYTlúœ<ÖVÂécÜÎ/_aãà¤EZ^·¸ûàßn×Øit@3PP—ÍÃ¾-ëZ¼Ô´Êô„WÚþð Û··Ñã^}V±é{�Zn²{Ü×ËË×—yØ�<ÐÈi´[‹ã‡ýz»ÿúKíý0>î _¾.ó®ØŸüz½›KNV…2õ  5£<î°œÏïn¿ß‰›>�Ãj”ãGYß_ß®áõð1ÍxyyY»Ð>åõííÿÊî¡óÖ¦€Âµ×Çä·õ²hˆt j[¬Ûæò~~†®‡y±©!Öî‚ñ¡ãåöúªÃ¾—G›>ƒÃ*Ë0GËçûÚ…1œ†Ã2ß^m
ªLŸ@ íÎ6~ý·û—›Ç“cV±ég  -ÏEwO�Ï¯ÏÏÙ’>t�{;¿��168%#ÅS—ë}Þ¼ùþøaœ//o÷„C¿âz9Ÿ¿Ú>E£H©wŸ\¾üx]Â®ï>|lÛÛÛ—e8ìU7§ß'�<W}øÔÛ�¯/ßÇ”î×ËõMwûƒkP€unšN‡²]_/ªû=\�ØÔƒ©mqþÃÞp;ÏÆ€£M#(b-oÉR¼-o[†»‡ƒ,—×óÍ�;GŠØô7AˆÙ¬è0ÏßÞkµj•é€AYE?G·^¾bûzÍNÅ¦Ÿ�AÞª†ïÂáËõëZãCï)J›~B¬ÝMú§>ØåòcÇ~÷ißã×çÿ—uvOâíýmvqï€LÊ*¡{êóvýQeØ�@¦? )íªéô9´¿zyA8ôý§ßØ½¼þúëy!Ÿ}{ýò>wc?>�CýòüõÓn÷yÿ¸l_^Ÿ1ÆÃã_?®/ß¾¼HÚu�»ä_ür	Ã0øG›ž@dË×¶û|ló·_ßÜn¿�b�œ>‚ZÎÅí>}Wñ—ïßÔÚn÷4°ÞÎ?,ÞwcN,›Äá0vÛõ¾&wLM)6� @7†#¸¼ÞW'ý°?øÛõùìF‘UÄ¨È¶�ñ1¬e�K;ÅH¡L;P f¥øýP±]ß«IßŸž†ÛýÛeõ–G¯ëó¥ºaç›Vh[Ö°ß™]ç{–ý …³é kÝúÓ±®o—u}wªjbSŠ˜Í®vãÃ“¾ßÎ%ï�~ƒØô±RyÄòrFŸM&«³2Cnëe-}ìÄ s�¹?�—ç{[Ó@gNl@‘¶esý©ÃåýõšÃ1ô„›<¬Í‡C'ey¿C¯¡:�¶®ý!1¿/—»��Î¦	Š°¶ì1ìƒ.ù‚æcg€µm2û~i—[KiØ“ÑVî*§Ã#nù|yŽ]èM •Â8žŒ·×‹­)yX½Qã‡žz}]‡1ÒTlA‘b«Óaïm]îÍbJ›¾Ej¹yÆCte9_	ßíN‡m¹\®ŒCL¾:ÀJ¶˜ö‡,ï—ó{NQE¦ß-×ê»!¹\®Ù¢õ§Ów ³†ýŽózeFt>Õé—°º.Ðý±ÛÞï·Œþc&6}ŠX©‹ÆSPn·M;ÐéoÃÚº†pØw¥½Ï¥E?:�–ë¦ÝaïóvYúÁ˜L{�%‡ÖíGgóõÙi?Ÿ|»¿]—9î"ÎX8·ß·ÝçÕé0(Dm:‚¬Õ˜bDµ�8¬Þc§.r»_q��gÓgPÄÚâó>«lÛ¶@RcB[n¹z„h¢ÓOÐêìsêA·|ÝDŽ.‰ˆ
[.~ŸJËï¥ ôÝnïk]_7bHÎÕéç K+ª�)p».Åï<U§ß…Õ\D÷ÉÑfÛBëÔSuú%¬.[ÀC×õ~¿%Ä(dÚƒukEÓnh%_7È)vEt:€m+k—†‡T.ok‰CLALÄ¦Ÿ€¨ÕjJ;ïçõ!ªx™ö°º”âv]Wr~Ÿ7•ÝiôcË·kî¬O‡7[ïÅrò§aôXïu	ñ™¾ƒÙº‰uqŒÛ|¾m®�ƒz¸éÃZË^Rðh%3˜s^�LÐJÝèáÖr-¦ÚíRWÛ\7ˆONE`¹UøqWd½çU}è¼ŠN{Ðê†æ¢wÍò]s'a7„¶å¼5¯šœˆêôû0ÛZuÝ¸Ût]×Ì¤�"ÈôS7ß´k£À3UÕéç0´\›¸!îîX/ÛÌ>õÝˆ’—m«^ƒ†æÛô‡ ÍJ3çv¾cÞò¶B¿cÚêmÙÊØ¢c4™þ#hmmÍ§!ö¹ÝV0DEU ÓŸ�´–ké˜œ¼oKK>x/NU¦ÿ ’fu•êÓNeYLÕyzS™~ÂØêfˆ1ŠŸ—ºAÕK„R´iðäV*½  (dê@¬fê]®ëFOuð^¢Ó¦ÀÒÌë!E±œ7ñê:ª:�þ3L`VK©ÁõfËÌÒÅ®ó¨y-Qà)B@¦¿ÆÌ7¤àÖ|U	D(Ælâ:—PË;VíRŸÚZ³ªŒn´Zg6×¢÷Á:ýw�0šeH¼ky¶ÜA’ÂÁ°YVßG•º.½)…š×-KŸ†Î—|o¯A"Eeú»0A«%‹î],u»oN¼ë-èô43¢ÕÚ\ç¶%çÉû^¨:}ZÍ:gµÎtE=U¦'Æ‚Í…>yYÎË,¾ïÚ\V‘q·µ¬·{¶Ô;eÝ°!ˆCˆƒLÿ$šµ�Îí‚wóönN5Z[1Ñ>µrm¦êU¡«rH»Úvk}?:µºlf}8õ	¥ÌlÔä ²¢VçSG¶;5‹s^§ïA³Z|T¢m†NûäX# >81¨ ÌØ2Í¥1€y­QUbÌjbJ�ºTá`á2=‚4«1‡�O!×[)N‚6ªˆNGA² {ßEA]7qê\Bj"ÓïÁ`0´ÜDw¡Wn-Ð«ÂA  i¤R-£jˆŠœ×‚èµ£oTÓ-†ä«Ì,à;ª‡èÔƒ†ªÕIŠ-ZØT|Óéw@ƒÕFD�Íqk•çT´¶±ºÔ{«·µ¸�º>4›óV]ÜwŽËÕsqº»,×¥Ê0¤˜(ëZÀÎ5”ºB½¨�~F¡Y1 ÞWÞÍ`*� )"ÓD#‹ŠÆØ¢m­á½ª˜À 
j­èbòÒ(¢.A¡2ý
$L ŠŠBõC–¥… ÒÁABˆM¿‰ÿ
ÝjNBASE      1è  xÁA  ƒ°YãxÔ þµ,¹†eÃ­-·Á¦)›f,µrYbŽU˜É1.ŒU˜É1.š+L�d�ÁÆ*L�d�e¦F²FŒ�‹Y…©‘¬cà"RVaj$kÄ¸h9¶Ø\·¶9� &cæ*Ö@—Œœ¶1¡A¬Zam¤ÛÔ¹éT·á\mP††3ZálVÐ´Z$Í`­¢&³Ú
�€	1I*˜TUb“2Vk«–&	-’Œ@@DD¬H\°H!‚"��°Ä0‚¤¹¤j˜8·\+’5ÄB'5„‰ÓÄmÌææ°1ZÐDæp£œ3*Œ1&D%š4—P…“adel+È˜M’�$r¹-«J7“%9f›{I’ŠBPOS      î  xÁA¶E±¯-êžìz ,œ�¦åÐM~ÿ÷ï___úñÓ½?îû=Þ¿ÿy¾m?-Ïuëõ}ï}žûëqïûÞ>÷ºç>|îÃ=¢í}Ôû(ï®µ^2m•èª÷xåbo+„hÞK�b­QYFA—:…5{›&ÐE#‹-aºhK\crÅ@ch‹¶fL[Y�™a€Ìb ²¶i³5lƒ ™`i`FƒÉ 3!6 1ƒƒ`€Ì3L`ÁXèŒFF` 2€Ì �A`€¦ 0 €À$‘Y@! BjÁDBZ4H,´`vkÖ†¦sk3X–m³¬ifÍšm³;œÜ0ð?7/óCNF4       ÷@  xÁANÂP€á¿”i¥`ª‘HªÆ@LdÁÜz�9•'ðÅS°pÕ…†Æ„R¬Ã{ã÷%o%èsÂÐÍHCö—à	iôññúgþy›ï²²íÏN]¼j/©—|,[˜�#‡ÑnL•
Ó_Z¨À¤©ô††Jïh(J}%¤:ÇD}a½Ò'Ö+}$�ê¡ÏûBsB�Ð!¢�V†]©CBO%DÄŒ¼-uH½ÀÌêÔç6µ\3z�ÑÄö¤%ƒ1¾f€}-ì´!”0>ÐNÚûnû` ý&²UGuÔQGuÔQGuô�8p³TEXT       Êó   xÁKŽ‚@Ð»”· ¨¼MÂ 
JÛð‘ šôˆÄ90bzý}F?D ÷c„	®çÓ_—Ež4õ—Jrª³²L–ZX×É7�Ü±Z¿D÷'©‘ßv«L*RV¡Ï›Žû“ÔÈo;É¡b©XIYóml¡¬Â™š3a¡””U8Ssr`aEû'taµÂx�.S±†vù3÷z?0øæ>œÓ©I…5Çpi{XaŒÐø]p�ß÷82Ç¸ÇYÑ§À?æø4{CLIP       	           +<AlignableSequences trimStringency="Medium"><Sequence name="37_TRE3GV-F1_TSS20210422-027-03401_B03" ID="0" isTrace="1" sortOrder="0" trimmedRange="14..998"/><Sequence name="36_TRE3GV-F1_TSS20210422-027-03401_A03" ID="1" isTrace="1" use="0" sortOrder="1" trimmedRange="21..998"/></AlignableSequences>  y‹     ÿ BC N srôe”```pðpáó³2Ô3ãö·²*Í+Î/*IMábÊqqQjZjQj^r*ÃL! ƒ2X6   ‹     ÿ BC í•=Ž1†ˆŸ‚Æ–%\°HŽR¤AÚ¥¡CÄJ:�8 Gà
¶R¸ä�C!qŒÅ|ÍîRP¯æ•<ñØ‘ÇÎHy.ž”?zû¸”‡×ðYú¿•º,?óù5­¦}¿(åêüþ)íÛ«RÆì¸`µ9H{ÅY­“×ðÕ}¬@¯æ´™šm¸‘ÑoFŒîìA¹q»1¿Ù>¦Í¶­­©Ú@‰z[›·º‡BTé±=cr�ZuV­m¢.±=Ø­Zë¼&£î°klsÖehÐwX(u3ž�À@Â$ž^ˆ€�rDH×¬²ö^±* ³ðânœ=	ØIËüt�²³Ž0CpNA¹è”„a,»9ÇFG�¾Ì–äÌˆ�MbI«Žs¨5}ŒíX‘NvU0FÎná0Xsá°–‚{tšnºÅi8só¹z„7uò<4¡þþúÐ¡C÷]¯Ï€xwÄó âª|~�`HÓ>ž×/o‚âòi)orýò¢”�ÑGÞÉþ—i0þâfø$0
–fdy@&² ùâ±< V&�Nß•ˆÑ¥µÕ‚)XÖ�nUy#(jæ&õ
:nâU¶ˆŽ6¢å A@©$R$®ö	@�Jhns%É(;–É’-´é9B�]–¸fÕ­0ºÿúísó‡R  ‹     ÿ BC             !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  -h<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2236" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2237-2249" color="#ffcc99" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2215-2233" color="#ff0000" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3142-3730" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4692" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="3901-4692" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4693-4761" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="2788-2794" color="#ffffff" type="standard"/><Segment range="2795-2812" color="#ffffff" type="standard"/><Segment name="-35" range="2813-2818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2833-2854" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2740-2756" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2764-2780" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1822-1943" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1385-1731" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1606-1624" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1570-1588" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1534-1552" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1498-1516" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1462-1480" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1426-1444" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1390-1408" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1738-1769" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ÷<?xml version="1.0"?><Primers nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="DF" sequence="CGCTGCTCTTTGAGCCTG" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1299-1316" boundStrand="0" annealedBases="CGCTGCTCTTTGAGCCTG" meltingTemperature="59"><Component hybridizedRange="1299-1316" bases="CGCTGCTCTTTGAGCCTG"/></BindingSite><BindingSite simplified="1" location="1299-1316" boundStrand="0" annealedBases="CGCTGCTCTTTGAGCCTG" meltingTemperature="59"><Component hybridizedRange="1299-1316" bases="CGCTGCTCTTTGAGCCTG"/></BindingSite></Primer><Primer recentID="1" name="R2" sequence="ATATTTTCTTGTTATAGATTAAGATACATTGATGAGTTTGGACAAAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1915-1961" boundStrand="1" annealedBases="ATATTTTCTTGTTATAGATTAAGATACATTGATGAGTTTGGACAAAC" meltingTemperature="60"><Component hybridizedRange="1915-1961" bases="ATATTTTCTTGTTATAGATTAAGATACATTGATGAGTTTGGACAAAC"/></BindingSite><BindingSite simplified="1" location="1915-1961" boundStrand="1" annealedBases="ATATTTTCTTGTTATAGATTAAGATACATTGATGAGTTTGGACAAAC" meltingTemperature="60"><Component hybridizedRange="1915-1961" bases="ATATTTTCTTGTTATAGATTAAGATACATTGATGAGTTTGGACAAAC"/></BindingSite></Primer><Primer recentID="2" name="R1" sequence="CAGCGTATCCACATAGCGTAGGTAGAATTCCATATGACGCGT" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1757-1798" boundStrand="1" annealedBases="CAGCGTATCCACATAGCGTAGGTAGAATTCCATATGACGCGT" meltingTemperature="69"><Component hybridizedRange="1757-1798" bases="CAGCGTATCCACATAGCGTAGGTAGAATTCCATATGACGCGT"/></BindingSite><BindingSite simplified="1" location="1757-1798" boundStrand="1" annealedBases="CAGCGTATCCACATAGCGTAGGTAGAATTCCATATGACGCGT" meltingTemperature="69"><Component hybridizedRange="1757-1798" bases="CAGCGTATCCACATAGCGTAGGTAGAATTCCATATGACGCGT"/></BindingSite></Primer><Primer recentID="3" name="Primer 3" sequence="TAAGATACATTGATGAGTTTGGACAAAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1915-1942" boundStrand="1" annealedBases="TAAGATACATTGATGAGTTTGGACAAAC" meltingTemperature="56"><Component hybridizedRange="1915-1942" bases="TAAGATACATTGATGAGTTTGGACAAAC"/></BindingSite><BindingSite simplified="1" location="1915-1942" boundStrand="1" annealedBases="TAAGATACATTGATGAGTTTGGACAAAC" meltingTemperature="56"><Component hybridizedRange="1915-1942" bases="TAAGATACATTGATGAGTTTGGACAAAC"/></BindingSite></Primer><Primer recentID="4" name="F2" sequence="TACGCTATGTGGATACGCTG" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1779-1798" boundStrand="0" annealedBases="TACGCTATGTGGATACGCTG" meltingTemperature="57"><Component hybridizedRange="1779-1798" bases="TACGCTATGTGGATACGCTG"/></BindingSite><BindingSite simplified="1" location="1779-1798" boundStrand="0" annealedBases="TACGCTATGTGGATACGCTG" meltingTemperature="57"><Component hybridizedRange="1779-1798" bases="TACGCTATGTGGATACGCTG"/></BindingSite></Primer><Primer recentID="5" name="Primer 1" sequence="ATCTATAACAAGAAAATAT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1943-1961" boundStrand="0" annealedBases="ATCTATAACAAGAAAATAT" meltingTemperature="40"><Component hybridizedRange="1943-1961" bases="ATCTATAACAAGAAAATAT"/></BindingSite><BindingSite simplified="1" location="1943-1961" boundStrand="0" annealedBases="ATCTATAACAAGAAAATAT" meltingTemperature="40"><Component hybridizedRange="1943-1961" bases="ATCTATAACAAGAAAATAT"/></BindingSite></Primer><Primer recentID="6" name="F1" sequence="GGCCTCCAAGGCCTACTAGTCCCTTTCGTCTTCACTCGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1344-1383" boundStrand="0" annealedBases="GGCCTCCAAGGCCTACTAGTCCCTTTCGTCTTCACTCGAG" meltingTemperature="71"><Component hybridizedRange="1344-1383" bases="GGCCTCCAAGGCCTACTAGTCCCTTTCGTCTTCACTCGAG"/></BindingSite><BindingSite simplified="1" location="1344-1383" boundStrand="0" annealedBases="GGCCTCCAAGGCCTACTAGTCCCTTTCGTCTTCACTCGAG" meltingTemperature="71"><Component hybridizedRange="1344-1383" bases="GGCCTCCAAGGCCTACTAGTCCCTTTCGTCTTCACTCGAG"/></BindingSite></Primer></Primers>
  �<Notes>
<UUID>7009c535-1e3b-4f92-8be2-7f0388db9375</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac-TRE3GV-MCS</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="10:22:59">2025.9.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO  P              E              ÿÿÿÿYô? P                                                                                                                                                              ©<StrandColors><TopStrand><ColorRange range="0..4760" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..4760" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
